Mesenchymal stem cell exosomes enhance the development of hair follicle to ameliorate androgenetic alopecia  

作　　者：Yu Fu Yao-Ting Han Jun-Ling Xie Rong-Qi Liu Bo Zhao Xing-Liao Zhang Jun Zhang Jing Zhang 

机构地区：[1]Research Center for Translational Medicine at East Hospital,School of Life Science and Technology,Tongji University,Shanghai 200092,China [2]Research Center for Translational Medicine at East Hospital,School of Medicine,Tongji University,Shanghai 200092,China [3]Tongji Lifeng Institute of Regenerative Medicine,Tongji University,Shanghai 200092,China [4]Research Center for Translational Medicine at East Hospital,Shanghai Institute of Stem Cell Research and Clinical Translation,School of Medicine,Tongji University,Shanghai 200092,China

出　　处：《World Journal of Stem Cells》2025年第3期102-123,共22页世界干细胞杂志(英文)

基　　金：Supported by the Peak Disciplines(Type IV)of Institutions of Higher Learning in Shanghai and the China Postdoctoral Science Foundation,No.2022M722409.

摘　　要：BACKGROUND Mesenchymal stem cells(MSCs)and their secretome have significant potential in promoting hair follicle development.However,the effects of MSC therapy have been reported to vary due to their heterogeneous characteristics.Different sources of MSCs or culture systems may cause heterogeneity of exosomes.AIM To define the potential of human adipose-derived MSC exosomes(hADSC-Exos)and human umbilical cord-derived MSC exosomes(hUCMSC-Exos)for improving dermal papillary cell proliferation in androgenetic alopecia.METHODS We conducted liquid chromatography-mass spectrometry proteomic analysis of hADSC-Exos and hUCMSC-Exos.Liquid chromatography-mass spectrometry suggested that hADSC-Exos were related to metabolism and immunity.Additionally,the hADSC-Exo proteins regulated the cell cycle and other 9 functional groups.RESULTS We verified that hADSC-Exos inhibited glycogen synthase kinase-3βexpression by activating the Wnt/β-catenin signaling pathway via cell division cycle protein 42,and enhanced dermal papillary cell proliferation and migration.Excess dihydrotestosterone caused androgenetic alopecia by shortening the hair follicle growth phase,but hADSC-Exos reversed these effects.CONCLUSION This study indicated that hair development is influenced by hADSC-Exo-mediated cell-to-cell communication via the Wnt/β-catenin pathway.

关 键 词：Mesenchymal stem cells EXOSOME Dermal papillary cells Hair development Liquid chromatography-mass spectrometry proteomic analysis 

分 类 号：R73[医药卫生—肿瘤]