六神曲在发酵过程中6种真菌毒素含量的动态变化规律分析  

Analysis of dynamic change patterns of six mycotoxin contents during the fermentation of Massa Medicata Fermentata

作　　者：王爽 周丽 时海燕[4] 赵霞 崔彦伟 包华音[1] 徐男[2] WANG Shuang;ZHOU Li;SHI Hai-yan;ZHAO Xia;CUI Yan-wei;BAO Hua-yin;XU Nan(College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Institute of Chinese Medicine Preparations,Shandong Provincial Academy of Traditional Chinese Medicine,Jinan 250014,China;State Key Laboratory for Regulation and Control Technology of Birth,Shandong Provincial Maternal and Child Health Care Hospital Affiliated to Qingdao University,Jinan 250014,China;Department of Clinical Pharmacy,The First Hospital Affiliated to Shandong First Medical University(Shandong Provincial Qianfoshan Hospital),Jinan 250014,China;Department of Research and Development,Shandong Baiweitang Traditional Chinese Medicine Slices Co.,Ltd.,Jinan 250108,China)

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]山东省中医药研究院中药制剂研究所,山东济南250014 [3]青岛大学附属山东省妇幼保健院,国家生育调控技术重点实验室,山东济南250014 [4]山东第一医科大学第一附属医院(山东省千佛山医院)临床药学部,山东济南250014 [5]山东百味堂中药饮片有限公司研发部,山东济南250108

出　　处：《中成药》2025年第3期740-744,共5页Chinese Traditional Patent Medicine

基　　金：山东省科技型中小企业创新能力提升工程(2022TSGC1057);山东省中医药科技项目重点项目(Z-2023012);山东省自然科学基金重点项目(ZR2020KH017);济南市科技型中小企业创新能力提升工程(2022TSGC1057);济南市“新高校20条”引进创新团队项目(202333021)。

摘　　要：目的分析六神曲在发酵过程中黄曲霉毒素B_(1)、黄曲霉毒素B_(2)、黄曲霉毒素G_(1)、黄曲霉毒素G_(2)、T-2毒素、脱氧雪腐镰刀菌烯醇含量的动态变化规律。方法UPLC-MS/MS分析采用Waters ACQUITY UPLC HSS T3色谱柱(100 mm×2.1 mm,1.8μm),流动相0.01%甲酸-[乙腈-甲醇(1∶1)],梯度洗脱;体积流量0.3 mL/min;柱温40℃;电喷雾离子源;正离子扫描;多反应监测模式。结果6种真菌毒素在各自范围内线性关系良好(R^(2)>0.9980),平均加样回收率76.1%~119.3%,RSD 0.49%~9.27%,其含量(除脱氧雪腐镰刀菌烯醇外)呈现从无到有、逐渐上升的趋势。结论六神曲在发酵过程中存在真菌毒素感染的风险。该方法简便高效,快速灵敏,可为全过程监测六神曲发酵工艺提供参考。AIM To analyze the dynamic change patterns of aflatoxin B_(1),aflatoxin B_(2),aflatoxin G_(1),aflatoxin G_(2),T-2 toxin and deoxynivalenol contents during the fermentation of Massa Medicata Fermentata.METHODS The analysis was performed on a 40℃thermostatic Waters ACQUITY UPLC HSS T3 column(100 mm×2.1 mm,1.8μm),with the mobile phase comprising of 0.01%formic acid-[acetonitrile-methanol(1∶1)]flowing at 0.3 mL/min,and electron spray ionization source was adopted in positive ion scanning with multiple reaction monitoring mode.RESULTS Six mycotoxins showed good linear relationships within their own ranges(R^(2)>0.9980),whose average recoveries were 76.1%-119.3%with the RSDs of 0.49%-9.27%,and except for deoxynivalenol,their contents demonstrated the trends of growing out of nothing and gradually increasing.CONCLUSION The risk of mycotoxin infection exists in the fermentation of Massa Medicata Fermentata.This simple,efficient,rapid and sensitive method can provide a reference for whole-process monitoring the fermentation process for Massa Medicata Fermentata.

关 键 词：六神曲 发酵 真菌毒素 含量测定 动态变化规律 UPLC-MS/MS 

分 类 号：Q815[生物学—生物工程]