Comparison of effects of calcium and chlorogenic acid intervention on bone and intestinal injury in cadmium-exposed growing rats  

作　　者：Yu-Na Kan Xiao-Wei Wang Hui-Fang Chen Xiao-Jiao Gao Xiao Huang Jia-Ming Xie 

机构地区：[1]School of Basic Medical Sciences/School of Public Health,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China [2]The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang 550001,China [3]School of Health Preservation,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China

出　　处：《Food and Health》2025年第2期24-34,共11页食品和健康(英文)

基　　金：supported by Guizhou Provincial Basic Research Program(Natural Science)(Qiankeheji-ZK[2021]General 497);Guizhou Health and Wellness Commission Science and Technology Fund Project(2024-247);Guizhou University of Traditional Chinese Medicine Academic Seedling Program(Guizhou Kehe Academic Seedling[2023]-35),The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine Institutional Research Grants(GZEYK[2020-34]).

摘　　要：Background:Cadmium(Cd)exposure disrupts bone microstructure and elevates osteoporosis risk,warranting innovative functional food development to alleviate its toxic effects.This study evaluated the protective effects of calcium(Ca)and chlorogenic acid(CGA)against Cd-induced bone damage,intestinal injury,and gut microbiota dysbiosis in rats.Methods:Thirty-two female Sprague-Dawley rats were allocated to four groups:control(Con),Cd-exposed(Cd),Cd+calcium gluconate(Ca),and Cd+CGA(CGA).Eight weeks after the experiment,histopathological analyses of bone and intestinal tissues,Cd content measurements,and gut microbiota composition assessments were conducted.Concurrently,serum superoxide dismutase(SOD)activity was determined.Results:Cd exposure significantly increased serum SOD levels and bone Cd levels(P<0.05),which was significantly attenuated under Ca and CGA interventions,with CGA showing superior efficacy(P<0.05).Histological analysis revealed Cd-induced trabecular resorption and adipocyte proliferation in bone,which were ameliorated by both interventions.CGA administration significantly improved intestinal morphological parameters(villous height,crypt depth)and reduced Chiu scores(P<0.05),whereas Ca had limited impact.Gut microbiota analysis indicated Cd exposure induced microbial dysbiosis,characterized by elevatedα-diversity indices(Chao1 and Shannon),which Ca partially stabilized by enriching beneficial taxa(e.g.,Lactobacillaceae).Conversely,CGA reduced microbial diversity but enhanced barrier-related metabolic pathways(e.g.,nucleotide biosynthesis).Both Ca and CGA interventions effectively reduced Proteobacteria abundance and partially restored the Firmicutes/Bacteroidetes(F/B)ratio,However,genus-level responses diverged significantly:Ca specifically enriched Blautia;CGA,in contrast,promoted Bifidobacterium and Oscillospira.Conclusion:Ca and CGA differentially protect against Cd toxicity.Integrating Ca-fortified foods(via competitive inhibition of Cd absorption)with CGA-rich extracts(via antioxidant activity a

关 键 词：CADMIUM CALCIUM chlorogenic acid bone microstructure gut microbiota oxidative stress 

分 类 号：R73[医药卫生—肿瘤]