酶定向改造及其在基因检测中的研究进展  

Research progress on enzyme-directed medication and its application in gene detection

作　　者：彭荣俞 蒋析文 PENG Rongyu;JIANG Xiwen(School of Chemistry and Chemical engineering,Guangdong Pharmaceutical University,Guangzhou,Guangdong,China,528458;The Research Institute of Daan Gene Co.,Ltd.,Guangzhou,Guangdong,China,510665)

机构地区：[1]广东药科大学医药化工学院,广东广州528458 [2]广州达安基因股份有限公司研究院,广东广州510665

出　　处：《分子诊断与治疗杂志》2025年第3期585-588,共4页Journal of Molecular Diagnostics and Therapy

基　　金：广州市科技计划项目(2024B01J1277)。

摘　　要：作为自然界的生物催化剂,酶能够催化广泛的化学转化,不仅反应条件温和,而且活性和选择性高。然而,天然酶对非天然物质的催化活性或其对映体选择性的不足均限制了它们的工业应用,通过酶的定向改造可提高酶的催化活性和稳定性,同时提高可识别的底物范围。本文综述了酶的定向改造过程,包括突变文库的构建和筛选方法,以及酶定向改造的相关研究在基因检测领域的应用,通过对酶的定向改造,提高了基因检测的灵敏度、检测范围及检测条件。As natural biocatalysts,enzymes can catalyze a wide range of chemical transformations with mild reaction conditions,high activity,and selectivity.However,the lack of catalytic activity or enantiomeric selectivity of natural enzymes for non-natural substances limits their industrial applications.Directed evolution of enzymes,can improve the catalytic activity and stability of enzymes,while expanding the range of substrates that can be recognized.This article reviews the process of directed enzyme modification,including the construction and screening methods of mutation libraries,as well as the application of related research on enzyme directed modification in the field of genetic testing.Through directed modification of enzyme,the sensitivity,detection range,and detection conditions of genetic testing have been improved.

关 键 词：酶 生物催化 定向改造 高通量筛选 基因检测 

分 类 号：G63[文化科学—教育学]