Expression profiling and bioinformatics analysis of serum exosomal circular RNAs in lymph node metastasis of papillary thyroid carcinoma  

作　　者：Huiyong Peng Zhangwei Zhu Jie Xing Qian Xu Changfeng Man Shengjun Wang Yingzhao Liu Zhengdong Zhang 

机构地区：[1]Department of Environmental Genomics,Jiangsu Key Laboratory of Cancer Biomarkers,Prevention and Treatment,Collaborative Innovation Center for Cancer Personalized Medicine,School of Public Health,Nanjing Medical University,Nanjing,Jiangsu 211166,China [2]Department of Genetic Toxicology,the Key Laboratory of Modern Toxicology of Ministry of Education,Center for Global Health,School of Public Health,Nanjing Medical University,Nanjing,Jiangsu 211166,China [3]Department of Laboratory Medicine,Zhenjiang Medical School of Nanjing Medical University,Zhenjiang,Jiangsu 212002,China [4]Department of Endocrinology,Zhenjiang Medical School of Nanjing Medical University,Zhenjiang,Jiangsu 212002,China [5]Department of Oncology,Zhenjiang Medical School of Nanjing Medical University,Zhenjiang,Jiangsu 212002,China [6]Department of Laboratory Medicine,the Affiliated Hospital of Jiangsu University,Zhenjiang,Jiangsu 212008,China

出　　处：《Journal of Biomedical Research》2025年第2期155-170,I0001-I0004,共20页生物医学研究杂志(英文版)

基　　金：National Natural Science Foundation of China (Grant No. 81800698);Jiangsu Provincial Medical Key Discipline Cultivation Unit (Grant No. JSDW202241);Research Project of Jiangsu Commission of Health (Grant No. H2023053);Zhenjiang Science and the Technology Planning Project (Grant Nos. SH2023006and SH2023008)。

摘　　要：Most papillary thyroid carcinoma(PTC) patients have a good prognosis. However, lymph node metastasis(LNM), the most common manifestation of disease progression, is frequently associated with a poor prognosis.Nevertheless, few studies have focused on the underlying mechanisms of LNM. In the current study, we aimed to investigate the potential role of exosomal circRNAs that contribute to LNM in PTC. We identified 9 000 differentially expressed exosomal circRNAs in PTC patients with LNM, including 684 upregulated and 2 193 downregulated circRNAs. Functional enrichment analysis revealed that these differentially expressed circRNAs were primarily involved in a variety of molecular and signaling pathways correlated with PTC progression and LNM. Through bioinformatics analysis, we identified 14 circRNA-miRNA-mRNA networks related to LNM-associated signaling pathways in PTC. Moreover, both circTACC2-miR-7-EGFR and circBIRC6-miR-24-3p-BCL2L11 axes were verified for their potential involvement in PTC with LNM. Additionally, we identified four upregulated circRNA-related hub genes and eight hub genes correlated with downregulated circRNAs, some of which were validated as being potentially involved in LNM in PTC. Collectively, our findings provide a novel framework for an in-depth investigation of the function of dysregulated exosomal circRNAs and their potential as biomarkers in PTC patients with LNM.

关 键 词：papillary thyroid carcinoma EXOSOME circular RNA regulatory network lymph node metastasis 

分 类 号：R736.1[医药卫生—肿瘤]