Structure-based insights into fluorogenic RNA aptamers  

作　　者：Qianqian Song Xiaoqing Tai Qianyu Ren Aiming Ren 

机构地区：[1]Life Sciences Institute,Second Affiliated Hospital of Zhejiang University School of Medicine,Zhejiang University,Hangzhou 310058,China [2]Agricultural College,Yangzhou University,Yangzhou 225009,China

出　　处：《Acta Biochimica et Biophysica Sinica》2025年第1期108-118,共11页生物化学与生物物理学报(英文版)

基　　金：supported by the grants from the Natural Science Foundation of China(Nos.32325029,32022039,91940302,and 91640104);the National Key Research and Development Project of China(Nos.2023YFC2604300 and 2021YFC2300300).

摘　　要：Fluorogenic RNA aptamers are in vitro-selected RNA molecules capable of binding to specific fluorophores,significantly increasing their intrinsic fluorescence.Over the past decade,the color palette of fluorescent RNA aptamers has greatly expanded.The emergence and development of these fluorogenic RNA aptamers has introduced a powerful approach for visualizing RNA localization and transport with high spatiotemporal resolution in live cells.To date,a variety of tertiary structures of fluorogenic RNA aptamers have been determined using X-ray crystallography or NMR spectroscopy.Many of these fluorogenic RNA aptamers feature base quadruples or base triples in their fluorophore-binding sites.This review summarizes the structure-based investigations of fluorogenic RNA aptamers,with a focus on their overall folds,ligand-binding pockets and fluorescence activation mechanisms.Additionally,the exploration of how structures guide rational optimization to enhance RNA visualization techniques is discussed.

关 键 词：fluorogenic RNA aptamers tertiary structures base quadruples base triples fluorescence activation mechanisms G-QUADRUPLEX 

分 类 号：R73[医药卫生—肿瘤]