龙葵多酚提取工艺优化及其抗氧化活性分析  

Optimization of Polyphenol Extraction Process from Solanum Nigrum and Analysis of its Antioxidant Activity

作　　者：王小淑[1] 杨鹏飞 明静云 刘盛微 刘欣 张洪信 Wang Xiaoshu;Yang Pengfei;Ming Jingyun;Liu Shengwei;Liu Xin;Zhang Hongxin(Guangxi Science&Technology Normal University,Laibin Guangxi 546199,China)

机构地区：[1]广西科技师范学院,广西来宾546199

出　　处：《山西化工》2025年第3期17-22,共6页Shanxi Chemical Industry

基　　金：广西科技师范学院2024年度校级教师科研基金项目(GXKS2024ZD003);广西科技师范学院2024年度大学生科研基金项目(GXKS2023DXS033);广西科技师范学院2024年校级大学生创新训练项目(X2024366)。

摘　　要：以龙葵多酚提取率为指标,经单因素实验和响应面分析法优化其提取工艺,得到最佳工艺条件:乙醇体积分数50%、液料比45∶1(mL/g)、微波温度71℃、微波时间3 min,龙葵多酚提取率2.05%。并对龙葵多酚进行DPPH自由基清除能力实验和羟自由基清除能力实验,以此评价其抗氧化活性。实验结果表明,当龙葵多酚提取液质量浓度为0.036 9 mg/mL时DPPH自由基清除率可达85.01%,当质量浓度为0.589 4 mg/mL时羟自由基清除率56.37%,说明龙葵多酚具有一定的抗氧化活性,可为龙葵资源的开发利用提供参考价值。Taking the extraction rate of solanum nigrum polyphenols as the index,the extraction process was optimized through single factor experiments and response surface analysis.The optimal process conditions were obtained:ethanol volume fraction of 50%,solid-liquid ratio of 45:1(mL/g),microwave temperature of 71℃,microwave time of 3 min,and solanum nigrum polyphenol extraction rate of 2.05%.And DPPH radical scavenging ability and hydroxyl radical scavenging ability experiments were conducted on solanum nigrum polyphenols to evaluate their antioxidant activity.The experimental results showed that the DPPH radical scavenging rate could reach 85.01%when the mass concentration of Solanum nigrum polyphenol extract was 0.0369 mg/mL,and the hydroxyl radical scavenging rate was 56.37%when the mass concentration was 0.5894 mg/mL.This indicates that solanum nigrum polyphenols have certain antioxidant activity and can provide reference value for the development and utilization of solanum nigrum resources.

关 键 词：龙葵 多酚 微波辅助提取 抗氧化活性 响应面分析法 

分 类 号：R284.1[医药卫生—中药学]