蒙花苷靶向内凝集蛋白1改善糖尿病血管内皮功能障碍的作用及机制  

Linarin Ameliorates Aortic Endothelial Dysfunction in Diabetic Mice by Targeting Intelectin 1

作　　者：李自成 毛爱琴 石萧明 何冬旭 LI Zicheng;MAO Aiqin;SHI Xiaoming;HE Dongxu(School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;Wuxi School of Medicine,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]江南大学无锡医学院,江苏无锡214122

出　　处：《食品科学》2025年第8期170-177,共8页Food Science

摘　　要：利用链脲佐菌素构建糖尿病小鼠模型,研究内凝集蛋白1(intelectin 1,ITLN1)在糖尿病小鼠主动脉内皮细胞中的修复作用,并遴选出食源性ITLN1激动剂蒙花苷,进一步证实蒙花苷对内皮细胞的保护效应。结果表明,在糖尿病小鼠主动脉内皮细胞中ITLN1的表达下降(66.68±9.51)%,并且当在内皮细胞中敲低ITLN1后,活性氧的生成会随着ITLN1表达水平的降低而升高。在使用50 mg/kg剂量的蒙花苷灌胃糖尿病小鼠28 d后,小鼠主动脉内皮细胞ITLN1的表达升高,活性氧(reactive oxygen species,ROS)生成水平下降,炎症因子肿瘤坏死因子-α、白细胞介素-1β(interleukin-1β,IL-1β)、IL-6均呈一定程度的下调。进一步研究发现蒙花苷可以抑制烟酰胺腺嘌呤二核苷酸磷酸氧化酶4和核因子κB,从而降低ROS的生成和炎症因子的表达,改善血管内皮损伤。因此,本研究提出蒙花苷可以靶向ITLN1,从而缓解糖尿病血管内皮功能障碍。This study utilized streptozotocin to create a diabetic mouse model to investigate the role of intelectin 1(ITLN1)in repairing the aortic endothelial cells of diabetic mice,and it also identified linarin as dietary ITLN1 agonist and evaluated the protective effect of linarin on endothelial cells.Results showed that the expression of ITLN1 in the aortic endothelial cells of diabetic mice decreased by(66.68±9.51)%.When the ITLN1 gene was knocked down in endothelial cells,the production of reactive oxygen species(ROS)increased with the decline in ITLN1 expression.Subsequently,after administration of linarin at a dose of 50 mg/kg for 28 days,the expression of ITLN1 in the aortic endothelial cells increased compared to the diabetic control group,ROS levels decreased,and the inflammatory factors tumor necrosis factor-alpha(TNF-α),interleukin-1β(IL-1β),and IL-6 were downregulated to some extent.Furthermore,mechanistic studies revealed that linarin could inhibit the nicotinamide adenine dinucleotide phosphate oxidase 4 and NF-κB,thereby reducing ROS production and the expression of inflammatory factors,attenuating vascular endothelial injury.Thus,this study proposes that linarin can alleviate endothelial dysfunction in diabetes by targeting ITLN1.

关 键 词：蒙花苷 糖尿病 内凝集蛋白1 主动脉内皮损伤 氧化应激 

分 类 号：R151.1[医药卫生—营养与食品卫生学]