黄大茶多糖的结构表征及其对3T3-L1前脂肪细胞分化的抑制作用  

Structural Characterization of Large Yellow Tea Polysaccharides and Its Inhibitory Effects on the Differentiation of 3T3-L1 Preadipocytes

作　　者：徐珊 谢海 李敏妮 马妍 葛慧芳 李大祥[1,2] 谢忠稳 王红燕 XU Shan;XIE Hai;LI Minni;MA Yan;GE Huifang;LI Daxiang;XIE Zhongwen;WANG Hongyan(National Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization,School of Food and Nutrition,Anhui Agricultural University,Hefei 230036,China;Joint Research Center for Food Nutrition and Health of IHM,Anhui Agricultural University,Hefei 230036,China;Anhui Provincial Joint Construction Key Laboratory of Industrial New-style Tea Beverage Green Manufacturing,Anhui Agricultural University,Hefei 230036,China)

机构地区：[1]安徽农业大学食品与营养学院,茶树种质创新与资源利用全国重点实验室,安徽合肥230036 [2]安徽农业大学大健康研究院食品营养健康联合研究中心,安徽合肥230036 [3]安徽农业大学工业新茶饮绿色制造安徽省联合共建学科重点实验室,安徽合肥230036

出　　处：《食品工业科技》2025年第8期67-75,共9页Science and Technology of Food Industry

基　　金：国家自然科学基金青年科学基金项目(32302007);安徽农业大学引进和稳定人才科研资助项目(yj2020-64);大学生创新创业训练计划项目(S202310364219);安徽特有茶资源健康功效和品质形成的分子机制(19235026);国家自然科学基金区域创新发展联合基金项目(U19A2034);现代农业产业技术体系建设专项资金(CARS-19);大健康研究院食品营养健康联合研究中心专项资金资助(2023SJY02)。

摘　　要：本文探究黄大茶多糖的结构特征和降脂活性,为夏秋茶资源的开发利用提供依据。以霍山黄大茶为试验材料提取黄大茶多糖,对其结构进行表征,分别采用高效液相凝胶渗透色谱(High-performance gel-permeation chromatography,HPGPC)、离子色谱(Ion chromatography,IC)、气质联用色谱(Gas chromatography-mass spectrometry,GC-MS)、傅里叶红外光谱(Fourier transform infrared spectrometer,FTIR)、扫描电镜(Scanning electron microscope,SEM)等分析方法测定黄大茶多糖的纯度、分子质量、单糖组成、化学键组成以及糖苷键结构;同时基于3T3-L1前脂肪细胞构建成熟脂肪细胞分化模型,研究黄大茶多糖干预对3T3-L1细胞分化和脂滴累积的影响。结果表明,黄大茶多糖经水提醇沉以及DE-52纤维素阴离子交换层析柱和Sephadex G-100凝胶柱纯化分离获得LYP-W1组分,HPGPC、IC和GC-MS分析结果显示,LYP-W1的分子量为1.25×105 Da,单糖组成为Ara:Rha:Gal:Glc:Man:GalA:GlcA为22.22:8.20:27.59:14.88:5.90:4.09,糖苷键主要由Rhap-(→1、Araf-(1→、→2)-Rhap-(1→、Glcp-(1→、GlcpA-(1→、Galp-(→1、→5)-Araf-(1→、→2,4)-Rhap-(1→、→4)-Galp-(→1、→4)-GalpA-(1→、→4)-Glcp-(1→、→3,4)-Manp-(1→、→3,6)-Galp-(1→等连接方式构成。FTIR结合SEM分析结果显示,LYPW1中具有β-型吡喃糖环,表面光滑致密,呈不规则片层薄膜状分布。细胞实验结果显示,LYP-W1在50~200μg/mL浓度范围内呈剂量依赖性显著抑制3T3-L1细胞的分化并降低其脂质沉积(P<0.05),表明黄大茶多糖具有天然降脂的潜力。本研究可为其它茶多糖的提取、纯化及生物活性研究提供参考。To provide a basis for the development and utilization of summer and autumn tea resources,the structural characteristics and lipid-lowering activity of polysaccharides from large-leaf yellow tea were investigated.The polysaccharides were extracted from Huoshan large-leaf yellow tea,which structural characteristics including purity,molecular weight,monosaccharide composition,functional groups and glycosidic bond types were characterized by high performance gel permeation chromatography(HPGPC),ion chromatography(IC),gas chromatography-mass spectrometry(GC-MS),fourier transform infrared spectrometer(FTIR),and scanning electron microscope(SEM).Concurrently,a mature adipocyte differentiation model was established using 3T3-L1 pre-adipocytes to investigate the effects of large-leaf yellow tea polysaccharide intervention on the differentiation and lipid droplet accumulation in 3T3-L1 cells.A homogeneous polysaccharide fraction(LYP-W1)was obtained from large-leaf yellow tea by water extraction,ethanol precipitation,and purification using DE-52 cellulose and Sephadex G-100 chromatography.Structure analysis indicated that LYP-W1 was composed of Ara:Rha:Gal:Glc:Man:GalA:GlcA=22.22:8.20:27.59:14.88:5.90:4.09 with a molecular weight of 1.25×105 Da,and its repeat unit of the glycosidic linkages were consisted of Rhap-(→1,Araf-(1→,→2)-Rhap-(1→,Glcp-(1→,GlcA-(1→,Galp-(→1,→5)-Araf-(1→,→2,4)-Rhap-(1→,→4)-Galp-(→1,→4)-GalpA-(1→,→4)-Glcp-(1→,→4)→,→3,4)-Manp-(1→,and→3,6)-Galp-(1→.Meanwhile,FTIR and SEM analysis revealed that LYP-W1 containedβ-type pyranose rings,with a smooth and compact surface and an irregular lamellar film-like distribution.In addition,LYP-W1 at dose of 50~200μg/mL can significantly inhibit the differentiation of preadipocytes lipid deposition in 3T3-L1 cells in in a dose-dependent manner,which suggests that LYP-W1 has good lipid-lowering activity(P<0.05).These results provided a scientific basis for the extraction,purification,and investigation of the biological ac

关 键 词：黄大茶多糖 分离纯化 结构鉴定 降脂活性 

分 类 号：S571.1[农业科学—茶叶生产加工]