沙棘多糖对小鼠酒精性肝炎和肠道屏障功能的治疗研究  

Treatment of Hippophae rhamnoides Polysaccharides on Alcoholic Hepatitis and Intestinal Barrier Function in Mice

作　　者：李清[1] 马广礼[1] 翟艺恒 孟涛[2] LI Qing;MA Guangli;ZHAI Yiheng;MENG Tao(College of Medical and Health,Xuchang Vocational Technical College,Xuchang 461000,China;Henan University,Kaifeng 475000,China)

机构地区：[1]许昌职业技术学院医疗卫生学院,河南许昌461000 [2]河南大学,河南开封475000

出　　处：《食品工业科技》2025年第8期351-361,共11页Science and Technology of Food Industry

基　　金：河南省高等教育教学改革研究与实践项目(2024SJGLX0800、2024SJGLX0814)。

摘　　要：目的:本研究旨在探讨沙棘多糖(Hippophae rhamnoides Polysaccharides,HRP)对酒精性肝炎(Alcoholic Hepatitis,AH)的保护作用及其作用机制,特别是其对小鼠肝炎和肠道屏障损伤的影响。方法:通过建立小鼠酒精性肝炎模型,使用沙棘多糖(低剂量100 mg/kg、高200 mg/kg)对小鼠进行灌胃处理(1次/日),观察其对小鼠血浆生化指标、肝脏组织生化指标、肝脏及肠道组织病理形态学变化的影响。同时,分析沙棘多糖对小鼠肠道通透性及结肠组织炎症和肠道屏障功能的影响。结果:与模型组相比,沙棘多糖低、高剂量组小鼠血浆中的AST、ALT显著降低,ALB明显升高(P<0.01或P<0.05)。HRP低、高剂量组能显著降低血浆中TC、TG、LDL-C、FFA水平,并提高HDL-C水平(P<0.01或P<0.05)。肝脏组织中,HRP能显著降低TG与TC含量,减少促炎因子IL-6、IL-1β和TNF-α的水平,并提高肝脏SOD、GSH水平,降低MDA水平(P<0.01或P<0.05)。病理学检查发现,HRP能改善肝脏结构,减少脂肪变性和炎症细胞浸润。此外,HRP能显著降低血浆LPS和D-乳酸水平,改善肠道组织病理学变化,并提高紧密连接蛋白基因表达水平,降低结肠炎症因子表达(P<0.01或P<0.05)。结论:HRP对酒精性肝炎具有保护作用,能够改善小鼠的肝脏损伤和肠道屏障功能,降低肝脏脂质蓄积和炎症反应。Objective:To investigate the protective effects of sea buckthorn polysaccharides(Hippophae rhamnoides Polysaccharides,HRP)on alcoholic hepatitis(AH)and its underlying mechanisms,particularly its effect on the impairment of hepatitis and intestinal barrier in mice.Methods:A mouse model of alcoholic hepatitis was established,and the mice were administered with sea buckthorn polysaccharides(low dose of 100 mg/kg and high dose of 200 mg/kg)by gavage once daily.Effects of HRP on plasma biochemical parameters,liver tissue biochemical indicators,and pathological morphological changes in liver and intestinal tissues were observed.Additionally,the effect of HRP on intestinal permeability,inflammation in colon tissue,and intestinal barrier function was analyzed.Results:Compared with the model group,the levels of AST and ALT in plasma significantly decreased,while ALB increased significantly in the low and high dose HRP groups(P<0.01 or P<0.05).HRP at both low and high doses significantly reduced the levels of TC,TG,LDL-C,and FFA in plasma and increased the level of HDL-C(P<0.01 or P<0.05).In the liver tissue,HRP significantly reduced the contents of TG and TC,decreased the levels of pro-inflammatory factors IL-6,IL-1β,and TNF-α,and increased the levels of liver SOD and GSH,while reducing MDA levels(P<0.01 or P<0.05).Histopathological examination revealed that HRP improved liver structure,reduced fatty degeneration,and inflammatory cell infiltration.Furthermore,HRP significantly reduced plasma LPS and D-lactic acid levels,improved intestinal histopathological changes,increased the expression level of tight junction proteins,and decreased the expression of colonic inflammatory factors(P<0.01 or P<0.05).Conclusion:HRP has a protective effect on alcoholic hepatitis,improving liver damage and intestinal barrier function in mice,and reducing liver lipid accumulation and inflammatory response.

关 键 词：沙棘多糖 酒精性肝炎 肠道屏障功能 氧化应激 炎症因子 

分 类 号：TS201.4[轻工技术与工程—食品科学]