胃癌预后相关基因的生物信息学鉴定  

Bioinformatics identification of key genes associated with the prognosis of gastric cancer

作　　者：刘钊 刘睿 范海静 孙卉 吕静[2] 张真 LIU Zhao;LIU Rui;FAN Haijing;SUN Hui;LYU Jing;ZHANG Zhen(Radiotherapy Department,Qingdao Central Hospital,University of Health and Rehabilitation Sciences,Qingdao 266042,China)

机构地区：[1]康复大学青岛中心医院放射治疗一科,山东青岛266042 [2]青岛大学附属医院肿瘤科

出　　处：《精准医学杂志》2025年第1期60-64,共5页Journal of Precision Medicine

摘　　要：目的筛选和鉴定胃癌预后相关的关键基因和通路,探讨胃癌发生发展的机制。方法从基因表达数据库中下载胃癌基因表达谱芯片数据集(GSE2685、GSE29272),通过在线分析工具GEO2R对胃癌组织和正常组织中的差异表达基因(DEGs)进行筛选;通过基因功能注释数据库(DAVID)进行功能富集分析;运用交互基因检索工具数据库(STRING)和Cytoscape软件分析DEGs可编码蛋白之间的相互作用网络;利用在线生存分析数据库(KM-plotter)分析核心候选基因和胃癌患者预后的相关性;通过免疫组化染色验证候选基因在胃癌组织中表达情况,并分析与胃癌临床病理参数及预后的相关性;采用Cox风险回归模型分析患者总体生存率的影响因素。结果通过数据库筛选得到5个与肿瘤分期及预后相关的DEGs:COL1A1、COL1A2、COL3A1、COL5A1和TFF2;免疫组化染色结果显示,COL3A1的表达水平与患者年龄、肿瘤分化程度、脉管癌栓、淋巴结转移、肿瘤分期及血管浸润有关(χ^(2)=5.480~13.830,P<0.05);COL3A1蛋白在胃癌组织中的表达升高与胃癌患者预后(总体生存率)显著相关(P<0.05)。单因素分析显示,肿瘤脉管癌栓、病理分化程度、分期、COL3A1表达水平与患者的总体生存率显著相关(P<0.05);将单因素分析中具有统计学意义的指标纳入多因素分析,结果显示,COL3A1高表达与患者的总体生存率显著相关(P<0.05)。结论COL3A1可能是胃癌诊断和预后的潜在靶标。Objective To screen and identify key genes and pathways associated with the prognosis of gastric cancer,and to investigate the mechanisms of the development and progression of gastric cancer.Methods Microarray datasets(GSE2685,GSE29272)of gastric cancer gene expression profile were downloaded from the gene expression omnibus,and the online analytical tool GEO2R was used to screen for differentially expressed genes(DEGs)between gastric cancer tissue and normal tissue.The Database for Annotation,Visualization,and Integrated Discovery was used for functional enrichment analysis.The Search Tool for the Retrieval of Interacting Genes and Cytoscape software were used to analyze the interaction network of the proteins encoded by the DEGs.The online survival analysis database(KM-plotter)was used to investigate the association between core candidate genes and the prognosis of gastric cancer patients.Immunohistochemical staining was used to verify the expression of candidate genes in gastric cancer tissue and analyze their association with the clinicopathological parameters and prognosis of gastric cancer.The Cox proportional hazards regression model was used to analyze the influencing factors for the overall survival of patients.Results Database screening obtained five DEGs that were associated with tumor staging and prognosis,i.e.,COL1A1,COL1A2,COL3A1,COL5A1,and TFF2,and immunohistochemical staining showed that the expression level of COL3A1 was associated with tumor differentiation and staging,vascular tumor thrombus,lymph node metastasis,and vascular invasion(χ^(2)=5.480-13.830,P<0.05).The increase in the expression of COL3A1 in gastric cancer tissue was significantly associated with the prognosis(overall survival rate)of gastric cancer patients(P<0.05).The univariate analysis showed that vascular tumor thrombus,the degree of pathological differentiation,staging,and the expression level of COL3A1 were significantly associated with the overall survival rate of patients(P<0.05).The indicators with statistical significan

关 键 词：胃肿瘤 预后 COL3A1基因 基因表达 计算生物学 数据库 遗传学 

分 类 号：R735.2[医药卫生—肿瘤] R319.1[医药卫生—临床医学]