PITC-HPLC法测定不同产地黄芪中氨基酸及综合评价  

Determination of Amino Acids in Astragalus Membranaceus from Different Origins Using PITCHPLC Method and Comprehensive Evaluation

作　　者：高伟城[1] 王小平[1] 李鑫如 陈荣珠[1] 李珍[1] GAO Weicheng;WANG Xiaoping;LI Xinru;CHEN Rongzhu;LI Zhen(Pharmacy Department of Zhangzhou Health Vocational College,Zhangzhou 363000,China)

机构地区：[1]漳州卫生职业学院药学院,福建漳州363000

出　　处：《江西中医药大学学报》2025年第2期71-80,共10页Journal of Jiangxi University of Chinese Medicine

基　　金：漳州卫生职业学院校级科技类项目(ZWYMN202301);第三批国家级职业教育教师创新团队项目(教师函[2023]9号);漳州市科技特派员项目(漳财教指[2024]22号)。

摘　　要：目的:建立异硫氰酸苯酯柱前衍生化-高效液相色谱法(PITC-HPLC)测定黄芪中氨基酸的含量并对其进行综合评价,为黄芪资源开发利用提供参考。方法:采用Excsep^(TM)AAA色谱柱(4.6 mm×250 mm,5μm),流动相采用乙腈-水-0.1 mol/L的醋酸钠缓冲液进行梯度洗脱,流速为1.0 mL/min,柱温为40℃,检测波长为254 nm。对数据进行相关性分析、聚类分析及主成分分析,根据氨基酸比值系数法及理想模式,对不同产地黄芪进行营养价值分析。结果:17种氨基酸均呈良好的线性关系,不同产地黄芪均含有16种氨基酸,其含量存在显著性差异,均含有7种人类必需氨基酸和9种药用氨基酸;12个产地中有6个产地未检测到胱氨酸。相关性分析表明,药用氨基酸与总氨基酸、必需氨基酸呈显著正相关,但与甜味氨基酸不相关。以总氨基酸及药用氨基酸为变量,12个产地黄芪可分为4类。主成分分析显示,综合得分值排前3的产地为内蒙古莫旗、山西平顺、甘肃岷县。不同产地黄芪必需氨基酸与非必需氨基酸的比值为46.908%~58.850%,必需氨基酸百分含量为31.930%~37.048%,SRC值为32.653~66.977,其中SRC值排前3的产地为甘肃渭源、宁夏彭阳、甘肃武都。不同产地黄芪的必需氨基酸含量高于WHO/FAO提出的成人需求值。结论:所建方法可用于黄芪氨基酸的含量测定,不同产地黄芪质量差异显著,黄芪的营养价值更适合于成人。Objective:A pre column derivatization high-performance liquid chromatography method(PITC-HPLC)was established to determine and comprehensively evaluate the content of amino acids in Astragalus membranaceus to provide reference for the development and utilization of Astragalus membranaceus resources.Methods:Used Excsep^(TM)AAA chromatography column(4.6 mm×250 mm,5μm),mobile phase was acetonitrileis-water-0.1 mol/L sodium acetate buffer,gradient elution was performed,flow rate was1.0 mL/min,column temperature was 40℃,detection wavelength was 254 nm.Perform correlation analysis,cluster analysis,and principal component analysis was used;nutritional value analysis of Astragalus membranaceus from different regions based on amino acid ratio coefficient method and ideal mode.Results:Seventeen amino acids all exhibited a good linear relationship.Astragalus membranaceus from different regions contained sixteen types of amino acids,with significant differences in their content.They all contained seven essential amino acids for humans and nine medicinal amino acids;cysteine was not detected in six out of twelve production areas.Correlation analysis showed that medicinal amino acids were significantly positively correlated with total amino acids and essential amino acids,but there was no statistically significant correlation with sweet amino acids.Used total amino acids and medicinal amino acids as variables,Astragalus membranaceus from twelve different regions can be divided into four categories.The principal component analysis showed that the top three regions with comprehensive scores were Moqi in Inner Mongolia,Pingshun in Shanxi,and Minxian in Gansu.The ratio of essential amino acids to non essential amino acids in Astragalus membranaceus from different regions was 46.908%-58.850%,the percentage content of essential amino acids was 31.930%-37.048%,and the amino acid ratio coefficient value was 32.653-66.977.The top three regions with amino acid ratio coefficient values were Weiyuan in Gansu,Pengyang in Ningxia,and Wu

关 键 词：黄芪 氨基酸 柱前衍生 主成分分析 营养价值 

分 类 号：R284.2[医药卫生—中药学]