酶法提取头孢菌素C菌渣中蛋白质工艺优化  

Optimization of enzymatic extraction of protein from cephalosporin C fermentation residues

作　　者：赵会如 刘柯妤 张立美 田甜甜 张继冉[1] 徐淑霞[1] 张世敏[1] 吴坤[1] ZHAO Huiru;LIU Keyu;ZHANG Limei;TIAN Tiantian;ZHANG Jiran;XU Shuxia;ZHANG Shimin;WU Kun(College of Life Sciences,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]河南农业大学生命科学学院,河南郑州450046

出　　处：《河南农业大学学报》2025年第2期266-275,共10页Journal of Henan Agricultural University

基　　金：河南省重点研发专项(221111112300);河南省科技攻关项目(222102110120);河南农业大学高层次人才专项支持基金项目(30501469)。

摘　　要：【目的】探究酶法高效提取头孢菌素C菌渣中蛋白质的工艺条件,拓展抗生素菌渣资源化利用途径。【方法】以蛋白质提取率为指标,在溶菌酶、蜗牛酶、酸性蛋白酶、中性蛋白酶、碱性蛋白酶和木瓜蛋白酶6种酶最适条件下,对头孢菌素C菌渣蛋白质进行提取,确定最适提取酶;考察酶添加质量分数、温度、时间、pH值和V(酶液/mL)∶m(头孢菌素C菌渣/g)(以下简称液固比)对头孢菌素C菌渣中蛋白质提取的影响,通过响应面试验优化确定最佳提取工艺条件。【结果】溶菌酶酶法提取头孢菌素C菌渣中蛋白质的效果最好,且主要受时间、pH值、液固比影响,影响顺序由高到低依次为液固比、时间、pH值。利用溶菌酶提取头孢菌素C菌渣蛋白质的最佳工艺条件为液固比7.0∶1.0,时间99 min,pH值7.2,酶添加质量分数1.0%,温度35℃,在此条件下头孢菌素C菌渣中蛋白质提取率最高,为69.06%。【结论】溶菌酶酶法能够快速、有效地提取菌渣中的蛋白质,促进头孢菌素C菌渣综合利用。【Objective】To study the process conditions for the efficient extraction of proteins from cephalosporin C residues by enzyme method,expanding the resource utilization of antibiotic residues.【Method】Under the optimum conditions of six enzymes,lysozyme,snail enzyme,acidic protease,neutral protease,alkaline protease and papain,the protein of cephalosporin C residues was extracted,and the optimum enzyme was determined used on the protein extraction rate;The effects of enzyme addition mass fraction,temperature,time,pH value and V/mL∶m(cephalosporin C residues/g)(liquid-solid ratio)on the extraction of the protein in cephalosporin C residues were investigated,and the optimum conditions of extraction were determined by optimization of the response surface test.【Result】Lysozyme was the optimum enzyme for protein extraction from cephalosporin C fermentation residues.The protein extraction efficiency was mainly affected by three factors in the order of liquid-solid ratio,time and pH value.The optimal process conditions for protein extraction were as follows:liquid-solid ratio of 7.0∶1.0,extraction time of 99 min,pH value 7.2,enzyme amount of 1.0%,temperature of 35℃,and the highest protein extraction rate of 69.06%was achieved under this condition.【Conclusion】Lysozyme is suitable for enzymatic hydrolysis and comprehensive utilization of cephalosporin C fermentation residues,which can quickly and effectively extract the proteins from bacterial fermentation residues.

关 键 词：头孢菌素C 抗生素菌渣 溶菌酶 蛋白质提取 资源化利用 

分 类 号：S141[农业科学—肥料学] X787[农业科学—农业基础科学]