东北9种浆果生物活性物质及抗氧化、抗炎活性比较  

Comparison on the Bioactive Substances,Antioxidant Capacities and Anti-inflammatory Activities of 9 Kinds of Berries in Northeast China

作　　者：张雅男 包怡红 王金玲 ZHANG Ya'nan;BAO Yihong;WANG Jinling(College of Life Science,Northeast Forestry University,Harbin 150040,China;Key Laboratory of Forest Food Resources Utilization of Heilongjiang Province,Harbin 150040,China)

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040 [2]黑龙江省森林食品资源利用重点实验室,黑龙江哈尔滨150040

出　　处：《现代食品科技》2025年第3期44-53,共10页Modern Food Science and Technology

基　　金：国家重点研发计划项目(2022YFD1600500);中央高校基本科研业务费专项(2572022DX05)。

摘　　要：为促进东北地区浆果相关产品的开发与利用,以东北9种种植范围广且常见的浆果为研究对象,测定分析浆果中总酚、总黄酮和总花色苷含量,通过高效液相色谱法测定浆果中酚类单体的种类和含量;通过测定DPPH自由基清除能力、ABTS~+自由基清除能力、透明质酸酶抑制能力和抑制白蛋白变性能力评价浆果抗氧化活性和抗炎活性,并进行相关性分析。结果显示,红豆越橘和蓝靛果中总酚、总黄酮和总花色苷含量较高,分别为2.83和2.42 g GAE/100 g DW、0.99和2.09 g Rutin/100 g DW、104.33和414.73 mg/100 g DW,且二者具有较强的抗氧化活性和抗炎活性。相关性结果表明,总酚含量决定浆果的抗氧化活性和抗炎活性。在9种浆果中鉴定并定量19种单体酚,绿原酸、隐绿原酸、熊果苷和黄岑素均在浆果中检出,绿原酸和隐绿原酸是大部分浆果中主要的酚类单体,红豆越橘中单体酚种类最多,蓝靛果中单体酚含量最高。该实验为东北浆果的综合利用提供理论依据。In order to promote the development and utilization of berry-related products in Northeast China,9 kinds of berries widely cultivated and common in Northeast China were used as the research objects,the contents of total phenols,total flavonoids and total anthocyanins in were determined and analyzed.The types and contents of the phenolic monomers in these berries were determined by high performance liquid chromatography.The antioxidant activity and anti-inflammatory activity of the berries were evaluated by measuring DPPH·scavenging ability,ABTS+∙scavenging ability,hyaluronidase inhibitory ability and albumin denaturation inhibitory ability,and correlation analysis was conducted.The results showed that the contents of total phenols,total flavonoids and total anthocyanins in blue honeysuckle and lingonberry were higher,which were 2.83 and 2.42 g GAE/100 g DW,0.99 and 2.09 g Rutin/100 g DW,104.33 and 414.73 mg/100 g DW,respectively.These two berries had strong antioxidant and anti-inflammatory activities.The correlation results showed that the total phenolic content determined the antioxidant and anti-inflammatory activities of the berries.19 monomer phenols were identified and quantified in 9 berries,with chlorogenic acid,cryptochlorogenic acid,arbutin and baicalein all being detected in berries.Chlorogenic acid and cryptochlorogenic acid were the main phenolic monomers in most berries.Among them,the number of the type of monomer phenol species in lingonberry and the content of the monomer phenols in blue honeysuckle were the highest.This experiment provides a theoretical basis for the comprehensive utilization of berries in Northeast China.

关 键 词：浆果 生物活性物质 抗氧化活性 抗炎活性 酚类单体 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]