酿酒葡萄渣微晶纤维素制备工艺优化及表征  

Preparation Process Optimization and Characterization of Microcrystalline Cellulose from Grape Pomace

作　　者：李睿娇 邱继尧 王伟 张磊[2] LI Ruijiao;QIU Jiyao;WANG Wei;ZHANG Lei(College of Food Science and Pharmacy,Xinjiang Agricultural University,Urumqi 830052,China;College of Forestry and Landscape Architecture,Xinjiang Agricultural University,Urumqi 830052,China)

机构地区：[1]新疆农业大学食品科学与药学学院,新疆乌鲁木齐830052 [2]新疆农业大学林学与风景园林学院,新疆乌鲁木齐830052

出　　处：《现代食品科技》2025年第3期290-299,共10页Modern Food Science and Technology

基　　金：新疆乡村振兴产业发展科技行动项目(2022NC030)。

摘　　要：为开发利用酿酒葡萄渣(Grape Pomace, GP)资源,创造经济价值,提升生态效益,该文以GP为原料,通过酸、碱、漂白处理纯化纤维素,基于单因素和Box-Behnken响应面试验,建立并优化葡萄渣微晶纤维素(Grape Pomace Microcrystalline Cellulose, GPMCC)制备工艺并对其进行表征。结果表明:制备GPMCC的最佳工艺为酸解时间74 min、酸解温度64℃、盐酸浓度3 mol/L、料液比1:20 (g/mL),此优化工艺下得率为81.52%,结晶度为75.07%,粒径分析表明,与GP相比,颗粒尺寸最高减小了50.19%,并且分布均匀;傅立叶变换红外光谱分析表明,纯化和酸解过程,有效去除了杂质;X射线衍射表明,通过逐步处理,GPMCC晶型结构仍为纤维素Ⅰ型,结晶度为75.07%;热重分析结果表明,GPMCC的热降解温度为235℃,明显高于GP,具有较好的热稳定性;GPMCC与市售微晶纤维素具有相似的结构性质。该研究成功的从GP中制备出GPMCC,为GP的增值利用提供了重要理论依据。Microcrystalline cellulose was prepared from grape pomace(GP),aiming to develop and utilize GP resources,create economic value,and improve ecological benefits.The prepared grape pomace microcrystalline cellulose(GPMCC)was purified by acid,alkaline,and bleach treatments.The GPMCC preparation process was established based on single-factor experimental design and Box-Behnken response surface design,and the prepared GPMCC was characterized.The results indicate that the optimal preparation process for GPMCC involves acid hydrolysis at 64℃for 74 min using a 3 mol/L hydrochloric acid solution and a solid-liquid ratio of 1:20 g/mL.The yield and crystallinity of GPMCC for this process were 81.52%and 75.07%,respectively.Particle size analysis revealed that the particle size of GPMCC was 50.19%smaller than that of GP,with a uniform distribution.Fourier-transform infrared spectroscopy confirmed the effective removal of impurities through purification and acid hydrolysis.X-ray diffraction revealed that GPMCC retained the cellulose type I crystal structure,with a crystallinity of 75.07%.Thermogravimetric analysis showed that GPMCC is more thermally stable than GP,as evidenced by its considerably higher thermal degradation temperature(235℃).Additionally,GPMCC exhibits similar structural properties to commercial microcrystalline cellulose.The successful preparation of GPMCC from GP provides an important theoretical basis for the value-added utilization of GP.

关 键 词：葡萄渣 微晶纤维素 响应面法 制备 结构表征 

分 类 号：TS201.2[轻工技术与工程—食品科学]