暗褐脉柄牛肝菌水提物脱色脱蛋白工艺优化及抗氧化活性  

Optimization of Decolorization and Deproteinization Process of Water Extract from Phlebopus portentosus Fruiting Bodies and Its Antioxidant Activity

作　　者：潘章超 韦衍瑜 王勇[1] PAN Zhangchao;WEI Yanyu;WANG Yong(Engineering Research Center of Tropical Medicine Innovation and Transformation of Ministry of Education,Hainan Provincial Key Laboratory of Research and Development on Tropical Herbs,School of Pharmacy,Hainan Medical University,Haikou 571199,Hainan,China)

机构地区：[1]海南医科大学药学院,热带药物创新与转化教育部工程研究中心,海南省热带药用植物研究开发重点实验室,海南海口571199

出　　处：《食用菌学报》2025年第2期60-71,共12页Acta Edulis Fungi

基　　金：海南省自然科学基金高层次人才项目(820RC623);海南医科大学研究生创新科研课题(HYYB2022A13)。

摘　　要：以脱色率、多糖保留率、脱蛋白率和综合评分为指标,筛选用于暗褐脉柄牛肝菌(Phlebopus portentosus)水提物(AEPP)脱色脱蛋白的大孔树脂;在单因素实验基础上,采用响应面法优化AEPP的脱色脱蛋白工艺,测定AEPP处理前后的理化特征及对ABTS、DPPH、超氧阴离子、羟基自由基的体外清除活性和铁原子还原力。结果表明:大孔吸附树脂HPD-100的脱色脱蛋白效果最佳,最佳工艺条件为大孔吸附树脂用量50%(m∶V)、振荡时间6.3 h、吸附温度75℃,在此条件下脱色率、多糖保留率、脱蛋白率和综合评分分别为73.53%、83.65%、99.89%和96.93%;脱色脱蛋白后AEPP对ABTS、DPPH、超氧阴离子、羟基自由基清除率的IC50值分别为7.40、4.08、0.37、0.42 mg·mL^(-1)。研究结果可为真菌水提物脱色脱蛋白工艺优化及暗褐脉柄牛肝菌开发提供参考。Using decolorization rate, polysaccharide retention rate, deproteinization rate and composite score as indices, macroporous adsorption resin was screened for removing color and protein from aqueous extract of Phlebopus portentosus(AEPP). Based on the results of single-factor experiments, a response surface design was used to optimize the decolorization and deproteinization process. The decolorized and deproteinized AEPP, D-AEPP, was compared with AEPP in terms of physical and chemical characteristics,scavenging activity against ABTS, DPPH, superoxide anion, and hydroxyl radicals in vitro, and ferric reducing antioxidant power. The results showed that the HPD-100 macroporous adsorption resin was the most effective for the decolorization and deproteinization of AEPP. The optimal process conditions were addition of 50%macroporous resin(m∶V), and then shaking at 75 ℃ for 6.3 h. Under these conditions, the decolorization rate,polysaccharide retention rate, protein removal rate, and the composite score were 73.53%, 83.65%, 99.89%,and 96.93%, respectively. The IC50 values of D-AEPP for scavenging ABTS, DPPH, superoxide anion, and hydroxyl radicals were 7.40, 4.08, 0.37, and 0.42 mg·mL^(-1), respectively. These results provided a reference for optimization of decoloration and deproteinization process for fungal aqueous extracts.

关 键 词：暗褐脉柄牛肝菌 水提物 脱色 脱蛋白 抗氧化 

分 类 号：TS201.2[轻工技术与工程—食品科学]