阿魏酸酯酶与植物乳杆菌联合接种对杂交构树发酵品质的影响  

Effect of co-inoculation of feruloyl esterase and Lactiplantibacillus plantarum on fermentation quality of hybrid Broussonetia papyrifera

作　　者：吕家英 王义翔 侯国庆 张辉 LÜJiaying;WANG Yixiang;HOU Guoqing;ZHANG Hui

机构地区：[1]石河子大学动物科技学院,新疆石河子833000 [2]新疆生产建设兵团第七师农业科学研究所,新疆奎屯833200

出　　处：《饲料研究》2025年第5期121-125,共5页Feed Research

基　　金：南疆重点产业创新发展支撑计划(项目编号:2022DB017)。

摘　　要：试验旨在探索阿魏酸酯酶(FAE)与植物乳杆菌(Lactiplantibacillus plantarum)联合接种对杂交构树青贮发酵品质的影响。试验采用真空袋法调制杂交构树青贮,共设置4个处理组,CK组(不添加菌酶制剂)、T1组(0.3%FAE)、T2组(0.2%植物乳杆菌)、T3组(0.3%FAE+0.2%植物乳杆菌)。发酵60 d后,测定各处理组的营养成分、发酵品质、微生物数量,并进行瘤胃体外产气试验。结果显示,T3组的杂交构树青贮中粗蛋白(CP)、可溶性糖(WSC)含量最高,中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)含量最低。T3组杂交构树青贮pH值、氨态氮/总氮(NH3-N/TN)含量、好氧菌(AB)和霉菌数量显著低于CK组(P<0.05),乳酸(LA)和乙酸(AA)含量显著增加(P<0.05)。T3组杂交构树青贮有机物消化率(OMD)、代谢能(ME)、理论最大产气量、产气速度显著高于其他组(P<0.05)。通过隶属函数结合青贮品质、发酵品质进行综合评定,T3组>T2组>T1组>CK组。研究表明,FAE与植物乳杆菌联合接种对杂交构树秆发酵品质改善效果较好,能够最大程度保留饲草的营养物质。This study aimed to explore the effects of the combined inoculation of feruloyl esterase(FAE)and Lactiplantibacillus plantarum on the silage fermentation quality of hybrid Broussonetia papyrifera.The silage was prepared using a vacuum bag method,and four treatment groups were set up,CK group(no microbial or enzymatic preparations),T1 group(0.3%FAE),T2 group(0.2%Lactiplantibacillus plantarum),and T3 group(0.3%FAE+0.2%Lactiplantibacillus plantarum).After 60 days of fermentation,the nutritional components,fermentation quality,and microbial counts of each treatment group were measured,and an in vitro rumen gas production test was conducted.The results showed that the contents of crude protein(CP)and soluble sugar(WSC)in T3 group were the highest,and the contents of neutral detergent fiber(NDF)and acid detergent fiber(ADF)were the lowest.The pH value,ammonia nitrogen/total nitrogen(NH3-N/TN)content,aerobic bacteria(AB)count,and mold count of the T3 group silage were significantly lower than those of the CK group(P<0.05),while the concentrations of lactic acid(LA)and acetic acid(AA)were significantly increased(P<0.05).The organic matter digestibility(OMD),metabolizable energy(ME),theoretical maximum gas production,and gas production rate of the T3 group silage were significantly higher than those of the other treatment groups(P<0.05).By using a membership function to comprehensively evaluate silage quality and fermentation quality,the order was T3 group>T2 group>T1 group>CK group.The study indicates that the combined inoculation of FAE and Lactiplantibacillus plantarum has the better effect on improving the fermentation quality of hybrid Broussonetia papyrifera,maximizing the preservation of the nutritional substances of the forage.

关 键 词：杂交构树 阿魏酸酯酶 植物乳杆菌 青贮 营养品质 瘤胃体外发酵 

分 类 号：S816[农业科学—饲料科学]