食品中真菌毒素免疫联检技术研究进展  

Research Progress on Immunoassay Techniques for the Simultaneous Detection of Mycotoxins in Food

作　　者：石亚坤 郎慧媛 倪保霞 轩志宏 刘洪美 叶金 王松雪 谢岩黎[1] SHI Ya-kun;LANG Hui-yuan;NI Bao-xia;XUAN Zhi-hong;LIU Hong-mei;YE Jin;WANG Song-xue;XIE Yan-li(College of Food Science and Engineering,Henan University of Technology,Zhengzhou 450001,China;Academy of National Food and Strategic Reserves Administration,NFSRA Key Laboratory of Grain and Oil Quality and Safety,Beijing 100037,China)

机构地区：[1]河南工业大学粮油食品学院,河南郑州450001 [2]国家粮食和物资储备局科学研究院,国家粮食和物资储备局粮油质量安全重点实验室,北京100037

出　　处：《分析测试学报》2025年第4期740-748,共9页Journal of Instrumental Analysis

基　　金：广西重点研发计划(桂科AB24010013);中央级公益性科研院所基本科研业务费专项资金(ZX2228);中国科协青年人才托举工程项目(2021QNRC001);国家自然科学基金(32202157)。

摘　　要：真菌毒素污染具有防控难、广泛性、痕量性和协同性的特点,已经成为全球食品安全领域研究的热点与焦点之一。多种真菌毒素混合污染的危害具有显著的协同性或加性效应,对人类及动物健康构成严重威胁。因此,相对于常规实验室分析方法,开发快速、灵敏、准确、能够同时检测多种真菌毒素的快速检测方法具有重要理论意义和应用价值。免疫分析技术由于操作简单、特异性强、反应速度快、成本低、对操作人员要求低而被广泛应用于真菌毒素的快速检测领域。该文综述了近5年基于免疫分析技术的真菌毒素多联检测技术的研究进展,介绍了食品中典型真菌毒素的概况、免疫分析的原理,归纳了8种多联检测方法的现状,并结合实际应用情况分析了各检测方法的优缺点,对其未来的发展趋势进行了展望,以期为食品中真菌毒素多联检测技术提供参考。Mycotoxin contamination is extensive,traceable,synergistic,and difficult to prevent and control,and has become one of the hot spots and focuses of global food safety research.The hazards of mixed contamination with multiple mycotoxins have significant synergistic or additive effects,posing a serious threat to human and animal health.As a result,in comparison to traditional laboratory analysis methods,developing a rapid detection method that is rapid,sensitive,accurate,and capable of detecting a variety of mycotoxins at the same time is of great theoretical significance and application value.Because of its simple operation,strong specificity,fast reaction speed,low cost,and low requirements for operators,immunoassay technology is widely used in the field of rapid detection of mycotoxins.This paper reviews the research progress of mycotoxin multiplexing detection technology based on immunoassay technology in the past five years,and introduces the general situation of typical mycotoxins in food and the principle of immunoassays.This paper summarizes the current situation of eight multiplexing detection methods,analyzes the advantages and disadvantages of each detection method combined with practical application,prospects its future development trend,and provides reference for the multiplexing detection technology of mycotoxins in food.

关 键 词：真菌毒素 食品安全 免疫分析 多联检测 

分 类 号：O657.3[理学—分析化学] R155.5[理学—化学]