预制菜中16种邻苯二甲酸酯类塑化剂的测定方法研究及污染水平分析  

Development and Application of a Method for Pollution Level Analysis of 16 Phthalate Plasticizers in Pre-prepared Dishes

作　　者：曾涛 杨荣[1] 李晓晶[1] 甘平胜[1] 刘苗[1] 卢祝靓子 李燕[1] 白志军[1] ZENG Tao;YANG Rong;LI Xiaojing;GAN Pingsheng;LIU Miao;LU Zhuliangzi;LI Yan;BAI Zhijun(Guangzhou Center for Disease Control and Prevention(Guangzhou Health Supervision Institute),Guangzhou 510440,China)

机构地区：[1]广州市疾病预防控制中心(广州市卫生监督所),广东广州510440

出　　处：《食品科学》2025年第9期329-336,共8页Food Science

基　　金：广州市卫生健康科技项目(20241A010053)。

摘　　要：建立一种高效、快捷地同时测定预制菜中16种邻苯二甲酸酯类塑化剂的气相色谱-串联质谱法,并对市售预制菜中16种邻苯二甲酸酯类塑化剂污染水平进行分析。结果表明,16种邻苯二甲酸酯类化合物在质量浓度0.02~2.00 mg/L范围内线性关系良好,决定系数(R^(2))均大于0.998,方法检出限为0.22~5.40μg/kg,低、中、高3个水平的加标回收率在79.6%~117.2%之间,相对标准偏差为0.6%~3.7%。在对100份市售预制菜样品的检测中发现,邻苯二甲酸二甲酯、邻苯二甲酸二丁酯(dibutyl phthalate,DBP)和邻苯二甲酸二(2-乙基)己酯(bis(2-ethylhexyl)phthalate,DEHP)检出率较高,分别为83.0%、91.0%和94.0%,检出最大值分别为334.7、1960.8、1603.3μg/kg,其中DBP和DEHP超标样品各1份。该检测方法前处理流程简单、空白控制低、回收率高,进而提高了分析速度和准确度,本研究可为预制菜产业食品安全风险和市场监管提供方法支撑和数据支撑。An efficient and rapid gas chromatography-tandem mass spectrometry(GC-MS/MS)method for the simultaneous determination of 16 phthalate plasticizers was established and applied to analyze the contamination levels of these phthalates in commercially available pre-prepared dishes.The results showed that a good linearity(R^(2)>0.998)was obtained for all analytes in the concentration range of 0.02-2.00 mg/L,and the limit of detection(LOD)of the method ranged from 0.22 to 5.40μg/kg.Recoveries at three spiked levels ranged from 79.6%to 117.2%with relative standard deviation(RSD)between 0.6%and 3.7%.In 100 samples,the detection rates of dimethyl phthalate(DMP),dibutyl phthalate(DBP)and bis(2-ethylhexyl)phthalate(DEHP)were 83.0%,91.0%and 94.0%at concentrations of up to 334.7,1960.8 and 1603.3μg/kg,respectively.Among these samples,two were found to exceed the standard limits for DBP and DEHP,respectively.The proposed method was characterized by simple pretreatment,low blank control,good recovery,rapid analysis speed and high accuracy.This study provides methodological and data support for food safety risk assessment and supervision in the preprepared dish industry.

关 键 词：预制菜 邻苯二甲酸酯类塑化剂 污染水平 气相色谱-串联质谱 

分 类 号：O657.63[理学—分析化学]