Effect of in vivo reprogramming of astrocytes combined with exercise training on neurorepair in rats with spinal cord injury  

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作  者:Zuliyaer Talifu Xin Xu Huayong Du Zehui Li Xiaoxin Wang Chunjia Zhang Yunzhu Pan Han Ke Wubo Liu Feng Gao Degang Yang Yingli Jing Yan Yu Liangjie Du Jianjun Li 

机构地区:[1]School of Rehabilitation,Capital Medical University,Beijing,China [2]Department of Spinal and Neural Functional Reconstruction,China Rehabilitation Research Center,Beijing,China [3]Chinese Institute of Rehabilitation Science,Beijing,China [4]Center of Neural Injury and Repair,Beijing Institute for Brain Disorders,Beijing,China [5]Beijing Key Laboratory of Neural Injury and Rehabilitation,Beijing,China [6]School of Population Medicine and Public Health,Chinese Academy of Medical Sciences/Peking Union Medical College,Beijing,China [7]Department of Neurology,Cheeloo College of Medicine,Shandong University,Qilu Hospital of Shandong University,Jinan,China [8]School of Health and Life Sciences,University of Health and Rehabilitation Sciences,Qingdao,China

出  处:《Animal Models and Experimental Medicine》2025年第4期595-605,共11页动物模型与实验医学(英文)

摘  要:Background:The inability of damaged neurons to regenerate and of axons to estab-lish new functional connections leads to permanent functional deficits after spinal cord injury(SCI).Although astrocyte reprogramming holds promise for neurorepair in various disease models,it is not sufficient on its own to achieve significant functional recovery.Methods:A rat SCI model was established using a spinal cord impactor.Seven days postsurgery,adeno-associated virus were injected to overexpress the transcription factors NeuroD1 and Neurogenin-2(Ngn2)in the spinal cord.The rats were then trained to walk on a weight-supported treadmill for 4 weeks,starting 14 days after modeling.The effects of these interventions on motor and sensory functions,as well as spinal cord tissue repair,were subsequently evaluated.Results:The combination of NeuroD1 and Ngn2 overexpression with weight-supported exercise training significantly improved gait compared to either inter-vention alone.The group receiving the combined intervention exhibited enhanced sensitivity in sensory assessments.Immunofluorescence analysis revealed increased colocalization of astrocytes and microtubule-associated protein 2-positive neurons in the injury area.These effects were more pronounced than those observed with spinal cord tissue repair alone.Additionally,the combined intervention significantly reduced glial scarring and the size of the injury area.Conclusion:Exercise intervention enhances the reprogramming effects of astrocytes and restores motor function,yielding better results than either intervention alone.

关 键 词:ASTROCYTE exercise training functional recovery REPROGRAMMING spinal cord injury 

分 类 号:R651.2[医药卫生—外科学]

 

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