后生元PostbioYDFF对铜绿假单胞菌的抑菌活性及其转录机制  

Inhibitory Activity and Transcriptional Mechanism of PostbioYDFF against Pseudomonas aeruginosa

作　　者：刘于倩 王铭 袁伟 臧金红 赵金山 李兆杰 胡瑞艳 彭传涛 LIU Yuqian;WANG Ming;YUAN Wei;ZANG Jinhong;ZHAO Jinshan;LI Zhaojie;HU Ruiyan;PENG Chuantao(College of Food Science and Engineering,Qingdao Agricultural University,Qingdao 266109,China;Shandong Technology Innovation Center of Special Food,Qingdao 266109,China;Qingdao Special Food Research Institute,Qingdao 266109,China;Navy Qingdao Special Recreation Center,Qingdao 266109,China)

机构地区：[1]青岛农业大学食品科学与工程学院,山东青岛266109 [2]山东省特种食品技术创新中心,山东青岛266109 [3]青岛特种食品研究院,山东青岛266109 [4]海军青岛特勤疗养中心,山东青岛266109

出　　处：《食品工业科技》2025年第9期185-195,共11页Science and Technology of Food Industry

基　　金：国家自然科学基金(32101917);青岛农业大学高层次引进人才项目(1122024)。

摘　　要：在本研究中,通过分析后生元PostbioYDFF对铜绿假单胞菌(P.aeruginosa)生物膜的影响以及P.aeruginosa超微结构和转录组的变化,研究了其对P.aeruginosa的抗菌活性及其作用机制,同时,为了验证转录组数据,进行了qPCR验证。结果表明,后生元PostbioYDFF对铜绿假单胞菌具有显著的抑菌活性,形成的抑菌圈直径为28.9±0.3 mm,MIC值为100μL/mL。此外,与对照组相比,后生元PostbioYDFF可以极显著抑制生物膜的形成,抑制率达到64.96%(P<0.01),可以显著清除已形成的生物膜,清除率达到30.66%(P<0.05)。还观察到后生元PostbioYDFF破坏了细菌结构并减弱了细菌运动性(游泳运动、集群运动和抽搐运动)。通过RNAseq结合qPCR验证,与对照组相比,后生元PostbioYDFF处理后共发现503个显著差异的基因,其中237个显著上调的基因主要富集在核糖体、RNA降解、氧化磷酸化等通路,266个显著下调的基因主要富集在能量代谢、脂质代谢、双组分系统等通路。本研究为后生元PostbioYDFF防控铜绿假单胞菌提供了重要的理论依据,并指出了在食品保鲜中的潜在应用。In the present study,the antibacterial activity of postbiotics PostbioYDFF against Pseudomonas aeruginosa(P.aeruginosa)and its mechanism of action were examined by analyzing its effects on biofilm formation and changes in the ultrastructure and transcriptome.Meanwhile,to validate transcriptome data,qPCR was conducted.The results showed that PostbioYDFF exhibited significant bacteriostatic activity against P.aeruginosa with a diameter of inhibition zone growth of 28.9±0.3 mm and the MIC value of 100μL/mL.In addition,compared with the control group,PostbioYDFF was able to significantly inhibit biofilm formation by 64.96%(P<0.01)and disrupted established biofilms by 30.66%(P<0.05).It was also observed that PostbioYDFF disrupted the bacterial structure and attenuated bacterial motility(swimming,swarming and twitching).Through RNA-seq analysis combined with qPCR validation,a total of 503 significantly different genes were found after PostbioYDFF treatment as compared with the control group,including 237 significantly up-regulated genes which were mainly enriched in ribosomal,RNA degradation,oxidative phosphorylation and other pathways,and 266 significantly down-regulated genes which were mainly enriched in the energy metabolism,lipid metabolism,and two-component system and other pathways.This study provides an important theoretical basis for the prevention and control of P.aeruginosa by PostbioYDFF,and points out a potential application in food preservation.

关 键 词：铜绿假单胞菌 后生元 转录组学 抑菌 防腐 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]