酒糟多酚类物质纯化及体外抗氧化活性研究  

Purification of polyphenols from distiller's grains and its antioxidant activity in vitro

作　　者：晏俊玲 郑佳 张伟建 李茂 赵佳伟 覃凤阳 YAN Junling;ZHENG Jia;ZHANG Weijian;LI Mao;ZHAO Jiawei;QIN fengyang(Technology Research Center of Wuliangye Distillery Co.,Ltd.,Yibin,Sichuan 644007,China;Key Laboratory of Wuliangye-flavor Liquor Solid-state Fermentation,China National Light Industry,Yibin,Sichuan 644000,China)

机构地区：[1]宜宾五粮液股份有限公司技术研究中心,四川宜宾644007 [2]中国轻工业浓香型白酒固态发酵重点实验室,四川宜宾644000

出　　处：《食品与机械》2025年第3期161-170,共10页Food and Machinery

摘　　要：[目的]纯化并富集具有良好抗氧化活性的酒糟多酚。[方法]采用有机溶剂萃取和大孔吸附树脂相结合的方法对酒糟深共熔溶剂提取液中的酚类物质进行富集回收,并测定纯化产物的纯度和抗氧化活性。对萃取剂种类及树脂类型进行筛选,通过吸附动力学、吸附等温线和热力学阐述吸附机理,并优化其最佳工艺条件;以清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基和2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)阳离子自由基的能力为指标,评价酚类物质的体外抗氧化活性。[结果]最适宜的萃取溶剂为二氯甲烷,当V_(二氯甲烷)∶V_(DES)为2∶1时,多酚回收率为90.83%;最适宜纯化酒糟多酚的树脂为DM130,其吸附率为78.70%。DM130树脂对酒糟多酚的吸附符合准二级动力学模型和Langmuir模型,吸附过程自发且放热。DM130树脂纯化酒糟多酚的最佳工艺条件为上样液p H 5、上样液质量浓度0.8 mg/m L、上样流速2 BV/h、上样体积3.4 BV、洗脱剂为60%乙醇、洗脱流速3 BV/h、洗脱剂体积6.9 BV。酒糟多酚深共熔溶剂提取液经二氯甲烷萃取和DM130树脂吸附总体回收率可达到88.90%,多酚纯度由原来的2.11%提高至16.05%。纯化产物中共检出7种酚类物质,其中阿魏酸含量最高。纯化后的多酚物质清除DPPH自由基和ABTS+自由基的半抑制质量浓度(IC50)值分别为0.0804,0.1850 mg/m L。[结论]试验建立的酒糟多酚纯化方法有效可行,且富集的酒糟多酚具有良好的抗氧化活性。[Objective]To purify and enrich the distiller's grain polyphenols(DGPs)with good antioxidant activity.[Methods]Organic solvent extraction combined with macroporous adsorption resin was used to enrich and recover DGPs from the distiller's grains deep eutectic solvent(DES)extraction solution.The purity and antioxidant activity of the purified products were determined.Extraction solvents and resin types were screened,and adsorption kinetics,isotherms,and thermodynamics were used to explain the adsorption mechanism.The optimal process conditions were determined.The in vitro antioxidant activity of the polyphenols was evaluated by measuring their ability to scavenge 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)free radicals and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt(ABTS)free radicals.[Results]Dichloromethane was the most suitable extraction solvent,with a polyphenol recovery rate of 90.83%when the ratio of dichloromethane to DES was 2∶1.The best resin for purifying DGPs was DM130,with an adsorption rate of 78.70%.The adsorption of DGPs by DM130 resin conformed to pseudo-second-order kinetics and the Langmuir adsorption model,indicating that the process is spontaneous and exothermic.The optimal purification conditions with DM130 resin were a sample solution pH of 5,a sample concentration of 0.8 mg/mL,a flow rate of 2 BV/h,a sample volume of 3.4 BV,and elution with 60%ethanol at a flow rate of 3 BV/h and an elution volume of 6.9 BV.The overall recovery rate of DGPs from the distiller's grains DES extraction solution after dichloromethane extraction and DM130 resin adsorption was 88.90%,with polyphenol purity increasing from 2.11%to 16.05%.Seven phenolic compounds were detected in the purified product,with ferulic acid being the most abundant.The IC50 values for scavenging DPPH and ABTS+free radicals were 0.0804 mg/mL and 0.1850 mg/mL,respectively.[Conclusion]The purification method for the DGPs established in this study is effective and feasible,and the enriched DGPs exhibit good antioxi

关 键 词：酒糟多酚 大孔吸附树脂 分离纯化 机理 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]