单核细胞增生李斯特氏菌、大肠埃希氏菌O157:H7、沙门氏菌三重荧光PCR检测方法的建立  

Establishment of Triple Fluorescence PCR Detection Method for Listeria monocytogenes,Escherichia coli O157:H7,and Salmonella

作　　者：劳嘉倩 蒋佳希 黄志深 尹玮璐 梁美丹 林秀敏 陈楷 LAO Jiaqian;JIANG Jiaxi;HUANG Zhishen;YIN Weilu;LIANG Meidan;LIN Xiumin;CHEN Kai(Guangzhou Institute for Food Inspection,Guangzhou 511400,China)

机构地区：[1]广州市食品检验所,广东广州511400

出　　处：《现代食品》2025年第5期216-222,共7页Modern Food

基　　金：广州市市场监督管理局科技项目(2023KJ36)。

摘　　要：为提高常见食源性致病菌单核细胞增生李斯特氏菌、大肠埃希氏菌O157:H7、沙门氏菌的检测效率,本研究与前期研制的LES共增菌培养基联用,参考《出口食品中食源性致病菌检测方法实时荧光PCR法》(SN/T 1870—2016)方法,通过优化反应体系及反应条件,建立一种多重荧光PCR检测3种目标菌的方法。通过验证不同混合比例共增菌培养物检测效果及体系的特异性、敏感性,成功构建3种目标菌“同平台共增菌-多重荧光PCR检测”方法,体系的特异性好,灵敏度高,能够为食源性致病菌的快速检测提供技术支持。In order to improve the detection efficiency of common foodborne pathogenic bacteria Listeria monocytogenes,Escherichia coli O157:H7 and Salmonella,this study was combined with the previously developed LES co-growth culture medium,referring to the SN/T 187—2016.By optimizing the reaction system and reaction conditions,a multiplex PCR method was established for the detection of three target bacteria.By verifying the detection effect of different mixed proportions of co-proliferating cultures and the specificity and sensitivity of the system,the“Co-proliferating with platform-multiple PCR detection”method for three target bacteria was successfully constructed.The system has good specificity and high sensitivity,and can provide technical support for the rapid detection of foodborne pathogens.

关 键 词：食源性致病菌 共增菌培养 多重荧光PCR检测 

分 类 号：TS207.4[轻工技术与工程—食品科学]