Stereoselective effects and its potential mechanism of ofloxacin on the growth and development of Rana nigromaculata:mainly liver lipid metabolism  

作　　者：Wenjun Zhang Miaomiao Teng Jin Yan 

机构地区：[1]Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes of Ministry of Education,College of Environment,Hohai University,Nanjing 210098,China [2]State Key Laboratory of Environmental Criteria and Risk Assessment,Chinese Research Academy of Environmental Sciences,Beijing 100012,China [3]National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology,School of Environmental Science and Engineering,Suzhou University of Science and Technology,Suzhou 215009,China

出　　处：《Frontiers of Environmental Science & Engineering》2025年第4期125-138,共14页环境科学与工程前沿(英文)

基　　金：supported by the National Natural Science Foundation of China(No.42207321);the Fundamental Research Funds for the Central Universities(China)(No.B230201006).

摘　　要：Most research on antibiotics in the environment disregards chiral antibiotics,such as ofloxacin(OF).In this study,tadpoles of Rana nigromaculata were exposed to 1μg/L OF and levofloxacin(LVFX,an enantiomer of OF)for 75 d.Compared with dextrofloxacin,LVFX treatment had a greater effect on the inhibition of bodyweight,body length,development stage,and pathological liver damage.Therefore,OF exerts a stereoselective inhibitory effect on both growth and development,which is consistent with the results at the systemic metabolism level.Transcriptomic analysis revealed that the differentially expressed genes between OF and LVFX were mainly immune related.Targeted metabolomics showed that the stereoselective biological effect of OF on R.nigromaculata was caused by differences in contents of PE-O 16:0-22:4,PE 16:0-14:0,TAG 45:0-FA16:0,PE-P 18:0-16:0,PE 16:0-16:0,PC 16:0-22:4+AcO,PE 18:1-18:3,PC 16:1-18:1+AcO,and PC 18:1-18:3+AcO.Furthermore,two enantiomers of OF were selectively bound to enzymes related to lipid metabolism.This study provides both theoretical and practical references for the accurate evaluation and scientific control of the ecological risk of chiral antibiotics.

关 键 词：Chiral antibiotics AMPHIBIANS Targeted metabolomics Molecular docking OBESITY 

分 类 号：X171.5[环境科学与工程—环境科学]