基于转录组学的茶树品种‘福建水仙’器官特异性分析  

Organ Specificity Analysis of Camellia sinensis'Fujian Shuixian'Based on Transcriptomics

作　　者：刘财国 于文涛 王鹏杰[1] 王泽涵 谷梦雅 叶乃兴[1] Liu Caiguo;Yu Wentao;Wang Pengjie;Wang Zehan;Gu Mengya;Ye Naixing(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,350002;Fujian Key Laboratory for Technology Research of Inspection and Quarantine,Technology Centre of Fuzhou Customs District PR,Fuzhou,350001)

机构地区：[1]福建农林大学园艺学院,福州350002 [2]福州海关技术中心,福建省检验检疫技术研究重点实验室,福州350001

出　　处：《分子植物育种》2025年第4期1140-1153,共14页Molecular Plant Breeding

基　　金：福建农林大学茶产业链科技创新与服务体系建设项目(K1520005A01);海关总署科研项目基金项目(2020HK-187);福建省科技计划引导性项目(2021N0024);福建张天福茶叶发展基金会科技创新基金项目(FJZTF01)共同资助。

摘　　要：为进一步探究茶树器官的功能特异性,本研究对茶树品种‘福建水仙’的不同器官进行转录组测序。结果显示:在芽、嫩叶、老叶、花、茎和根样品中分别获得117、114、85、505、168、540个特异表达基因。进一步对器官特异表达基因进行GO和KEGG功能富集分析,其中与蛋白质代谢相关的蛋白质输出途径在芽、嫩叶、老叶、茎和根中显著富集,包含SEC63、SEC61A、SRP72、SRPRB和SRP14等9个蛋白编码基因。泛素介导的蛋白水解途径在芽、花和根中显著富集,UBE3C、UBE2J1、UBE2N,UBE2D和UBE2G1等调控ubiquitin连接酶、泛素结合酶、自由基复合体和Cu17复合物。植物激素信号转导途径在根中显著富集,GID1和PYL分别调控赤霉素受体和脱落酸受体。本研究表明,蛋白质输出途径在叶片器官中表达并受到不同发育时期的影响,泛素介导的蛋白水解途径和植物激素信号转导途径可使茶树高效地改变蛋白质组分,调节胁迫反应,从而保证发育可塑性和环境适应性。In order to further explore the functional specificity of tea plant organs,this study conducts transcriptome sequencing on different organs of Camellia sinensis'Fujian Shuixian'.The results showed that 117,114,85,505,168 and 540 specific expression genes were obtained in buds,young leaves,old leaves,flowers,stems and roots,respectively.GO and KEGG functional enrichment analysis of organ-specific expression genes was further carried out.The protein output pathways related to protein metabolismwere significantly enriched in five samples,i.e.buds,young leaves,old leaves,stems and roots.These protein output pathways included nine protein coding genes such as SEC63,SEC61A,SRP72,SRPRB and SRP14.Ubiquitin-mediated proteolysis pathway was significantly enriched in buds,flowers and roots.UBE3C,UBE2J1,UBE2N,UBE2D and UBE2G1 regulated ubiquitin ligase,ubiquitin-binding enzyme,free radical complex and Cu17 complex.Plant hormone signal transduction pathway was significantly enriched in roots.GID1 and PYL reg ulated gibberellin receptor and abscisic acid receptor,respectively.The present study suggests that the protein export pathway is expressed in vegetative organs and affected by tissue aging.Ubiquitin-mediated proteolysis pathway and plant hormone signal transduction pathway could efficiently change protein components and regulate stress response in tea plants,thus ensuring developmental plasticity and environmental adaptability.

关 键 词：茶树 福建水仙 转录组 功能分析 特异表达 

分 类 号：S571.1[农业科学—茶叶生产加工]