化机浆预浸渍溶出物的抗氧化性及其在食品领域的应用前景  

Study on the Antioxidant Activity of Chemi-mechanical Pulp Pre-impregnated Residue and Its Application Prospect in Food Field

作　　者：焦婷 朱北平[1] 焦健[1] 苏晨 田庆文[1] 房桂干[1,3] 张凤山 邓拥军[1] JIAO Ting;ZHU Beiping;JIAO Jian;SU Chen;TIAN Qingwen;FANG Guigan;ZHANG Fengshan;DENG Yongjun(Jiangsu Key Lab of Biomass Energy and Material,Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources,Key Lab of Chemical Engineering of Forest Products,National Forestry and Grassland Administration,National Engineering Research Center of Low-carbon Processing and Utilization of Forest Biomass,Institute of Chemical Industry of Forestry Products,CAF,Nanjing,Jiangsu Province,210042;Nanjing Forestry University,Nanjing,Jiangsu Province,210037;Shandong Huatai Paper Co.,Ltd.,Dongying,Shandong Province,257335)

机构地区：[1]中国林业科学研究院林产化学工业研究所,江苏省生物质能源与材料重点实验室,江苏省林业资源高效加工利用协同创新中心,国家林业和草原局林产化学工程重点实验室,林木生物质低碳高效利用国家工程研究中心,江苏南京210042 [2]南京林业大学,江苏南京210037 [3]山东华泰纸业股份有限公司,山东东营257335

出　　处：《中国造纸学报》2025年第1期125-134,共10页Transactions of China Pulp and Paper

基　　金：国家自然科学基金面上项目(32271974);泰山产业领军人才工程专项经费(tscy20200213)。

摘　　要：本研究探讨了目前被当作废弃物的桉木化机浆预浸渍溶出物(PR)的抗氧化性及其在食品领域的应用潜力分析,探究了预浸渍条件(NaOH用量、温度和浆浓)对制得PR抗氧化性和得率的影响,研究了不同NaOH用量下制得PR的化学结构差异,建立了PR的组分结构与抗氧化性的构效关系,并以热稳定性、高温处理后抗氧化性和细胞毒性为评价指标,评估了PR在食品领域的应用性能。结果表明,NaOH用量是影响PR抗氧化性和得率的主要因素,NaOH用量越少,PR得率越低、抗氧化性越强。NaOH用量为0时,制得PR(R0)的抗氧化性最佳,DPPH自由基清除率最高可达82.63%。同时,R0具有良好的热稳定性,200℃下高温处理1 h后仍能保持初始抗氧化水平的84%,并且在100μg/mL浓度下对HepG2细胞无细胞毒性,满足食品领域应用的基本要求。醌式共轭结构是PR具有强抗氧化性的主要原因,NaOH用量过大(30 kg/t)时,制得PR中醌类物质含量显著降低,其抗氧化性也随之下降。Antioxidant activity of the eucalyptus chemi-mechanical pulp pre-impregnated residue(PR)recognized as waste was studied,and its application prospect in food field was explored.The effects of pre-impregnation conditions(NaOH dosage,temperature and pulp concen⁃tration)on the PR antioxidant activity and yield were investigated.The difference in the PR chemical structure at various NaOH dosage was studied.The conformational relationship between the PR composition structure and the antioxidant activity was established.The thermal sta⁃bility,antioxidant activity after high-temperature treatment and cytotoxicity were also used as the evaluation indexes to evaluate the applica⁃tion performance of PR in the food field.The results showed that the NaOH dosage was the main factor affecting the antioxidant activity and yield of the PR,and the less the NaOH dosage,the lower the yield and the stronger the antioxidant activity of PR.PR fabricated at NaOH dosage of 0(R0)had the best antioxidant activity with highest DPPH free radical scavenging rate of 82.63%.It was also found that R0 had good thermal stability,and could maintain 84%of the initial level after 1 h of thermal treatment at 200℃.Moreover,PR had no cytotoxicity to HepG2 cells at a concentration of 100μg/mL,which met the basic requirements for food applications.The quinone-type conjugate struc⁃ture was the main reason for strong antioxidant activity of PR.When the NaOH dosage was at a high level(30 kg/t),the content of quinone of PR decreased significantly,and thus led to a decreased antioxidant activity.

关 键 词：化学机械浆 预浸渍溶出物 抗氧化性 细胞毒性 食品应用 UPLC-MS 

分 类 号：TS79[轻工技术与工程—制浆造纸工程]