元蘑菌糠纤维素酶和木聚糖酶粗提工艺优化  

Optimization of Crude Extraction Process of Cellulase and Xylanase from Hohenbuehelia serotine Residue

作　　者：付晨曦 田烨 赵越龙 马诗雨 于博唯 杨佳琦 马怀良[1] Fu Chenxi;Tian Ye;Zhao Yuelong;Ma Shiyu;Yu Bowei;Yang Jiaqi;Ma Huailiang(College of Life Science and Technology Mudanjiang Normal University,Mudanjiang,Heilongjiang 157011)

机构地区：[1]牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157011

出　　处：《中国林副特产》2025年第2期13-14,共2页Forest By-product and Speciality in China

基　　金：牡丹江师范学院博士启动基金项目(MNUB201503);黑龙江省高校基本科研业务费科研项目(1354ZD006);牡丹江师范学院“揭榜挂帅”项目(kjcx2023-113mdjnu)。

摘　　要：为利用元蘑菌糠浸提纤维素酶和木聚糖酶,选取浸提温度20~40℃、时间0.5~4.0 h和液固比10∶1~50∶1为试验因素,以纤维素酶和木聚糖酶活力为指标,采用均匀设计对浸提工艺进行了优化。结果表明:最佳浸提条件是时间4.0 h,温度20℃,液固比50∶1。In order to extract cellulase and xylanase using Genghis Khania mushroom chaff,the extraction process was optimized with uniform design by selecting temperature 20~40℃,time 0.5~4.0 h and liquid-solid ratio 10∶1~50∶1 as the experimental factors,and cellulase and xylanase activities as the indicators.The results showed that the optimal extracting conditions were time 4.0 h,temperature 20℃and liquid-solid ratio 50∶1.

关 键 词：元蘑 纤维素酶 木聚糖酶 菌糠 

分 类 号：S646.9[农业科学—蔬菜学]