水稻香兰素内源性及生长阶段分布特征分析  

Analysis of endogenous and growth stage distribution characteristics of vanillin in Oryza sativa

作　　者：于娅 刘佳 张晓琳 杨红云 王健 陈修红 徐海青 杨丹 王浩杰 曹高峰 YU Ya;LIU Jia;ZHANG Xiao-Lin;YANG Hong-Yun;WANG-Jian;CHEN Xiu-Hong;XU Hai-Qing;YANG Dan;WANG Hao-Jie;CAO Gao-Feng(National Center for Quality Inspection and Testing of Non-stape Foods,International Trade Food Science Research Institute,Beijing 102209,China;Beijing Key Laboratory of Nutrition,Health and Food Safety,Quality and Safety Department,COFCO Nutrition and Health Research Institute Co.,Ltd.,Beijing 102209,China;Department of Quality and Safety Management,COFCO Group,Beijing 100020,China)

机构地区：[1]国家副食品质量检验检测中心,国贸食品科学研究院有限公司,北京102209 [2]营养健康与食品安全北京市重点实验室,中粮营养健康研究院有限公司,北京102209 [3]中粮集团有限公司质量安全管理部,北京100020

出　　处：《食品安全质量检测学报》2025年第9期92-98,共7页Journal of Food Safety and Quality

摘　　要：目的 研究水稻香兰素内源性及生长阶段分布特征。方法 选取龙粳46、盐丰和辽星作为供试材料,对水稻全生长周期的不同部位进行动态取样,并采用高效液相色谱-串联质谱方法测定香兰素含量。结果 香兰素在水稻苗期便已存在植株中,根部和叶片中的含量分别为1100~1450μg/kg和596~972μg/kg。在水稻生长过程中,根部含量在抽穗期达到峰值,为6560~8900μg/kg,从齐穗期至完熟期,根部香兰素含量逐渐降低,完熟期时含量降至2900~3230μg/kg;叶片中香兰素含量则在完熟期达到峰值;稻米中香兰素自腊熟期开始积累,至完熟期含量达到峰值。在生殖生长后期,植株上部(剑叶和茎上部)的香兰素含量高于植株下部(下部叶片和茎下部);稻壳中香兰素含量极显著高于糙米。结论 本研究首次明确证实水稻全生育期均有香兰素本底存在,确认香兰素为内源产生,其含量在不同部位和生育期呈现阶段性与差异性变化。推测其作用可能是水稻的天然生长调节剂。Objective To investigate the endogeneity and distribution characteristics of vanillin in different growth stages of Oryza sativa.Methods Longjing 46,Yanfeng,and Liaoxing were selected as the experimental materials.Samples were taken at different parts of Oryza sativa during its whole growth period.The vanillin content was determined by high performance liquid chromatography-tandem mass spectrometry method.Results Vanillin was present in Oryza sativa plants as early as the seedling stage.Its content in roots and leaves was 1100–1450 μg/kg and 596–972 μg/kg,respectively.During the growth process of Oryza sativa,the vanillin content in roots reached its peak at heading stage,with a content of 6560–8900 μg/kg.Subsequently,from full-heading stage to maturity stage,the vanillin content in roots gradually decreased,and at maturity stage,the content dropped to 2900–3230 μg/kg.The vanillin content in the leaves reached its peak at maturity stage.The vanillin in Oryza sativa grains started to accumulate continuously from the dough stage and reached its peak at maturity stage.In addition,at the late stage of reproductive growth,the vanillin content in the upper part of the plant(flag leaves and the upper part of the stem) was significantly higher than that in the lower part of the plant(lower leaves and the lower part of the stem),and the vanillin content in Oryza sativa husk was extremely significantly higher than that in the brown Oryza sativa.Conclusion This study confirms that vanillin has a natural background throughout the whole growth period of Oryza sativa,and vanillin is endogenously produced for the first time.Its content shows stage-specific and differential changes at different parts and growth stages.It is speculated that it may be a natural growth regulator of Oryza sativa.

关 键 词：水稻 香兰素 内源性 质量安全标准 天然合成 

分 类 号：S511[农业科学—作物学]