辐照-水热处理制备油茶壳低聚木糖的脱色工艺与抗氧化性研究  

Study on the decolorization process and antioxidant activity of xylo-oligosaccharides in Camellia oleifera shell prepared via irradiation-hydrothermal treatment

作　　者：沈晓岩 陈亮[2] 齐慧[2] 刘安 魏东宁 邓明[2] 王克勤 武小芬[1,2] SHEN Xiaoyan;CHEN Liang;QI Hui;LIU An;WEI Dongning;DENG Ming;WANG Keqin;WU Xiaofen(Longping Branch,College of Biology,Hunan University,Changsha 410125,China;Hunan Institute of Nuclear Agricultural Science and Space Breeding,Hunan Academy of Agricultural Sciences,Changsha 410125,China)

机构地区：[1]湖南大学生物学院隆平分院,长沙410125 [2]湖南省农业科学院/湖南省核农学与航天育种研究所,长沙410125

出　　处：《辐射研究与辐射工艺学报》2025年第2期72-85,共14页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金(12205094);湖南省重点研发项目(2023NK2040-3);湖南省农业科技创新资金(2023CX59)资助。

摘　　要：采用电子束辐照协同水热处理制备油茶壳低聚木糖,通过优化活性炭脱色工艺,测定并分析脱色后油茶壳低聚木糖的抗氧化活性,为油茶加工副产物高值化利用提供新途径。结果表明:油茶壳经3 MeV电子束辐照协同水热处理,制备所得的低聚木糖质量浓度为13.87 mg/mL,酶解后木二糖至木四糖的质量浓度分别为3.37 mg/mL、4.76 mg/mL、2.48 mg/mL。活性炭脱色优化工艺为:脱色温度30℃,脱色时间60 min,活性炭添加量50 mg/mL,pH=4;在该条件下Darco G60活性炭对油茶壳水热-酶解液的脱色率达到93.77%,此时木二糖至木四糖和总低聚木糖的保留率分别为97.66%和71.50%。经扫描电镜和红外光谱分析得知,油茶壳低聚木糖呈无定型结构,多部分相互交联,主链是由D-木糖通过β-1,4糖苷键连接而成。此外,当油茶壳低聚木糖质量浓度为10 mg/mL时表现出较好的抗氧化活性,其对2,2-联苯基-1-苦基肼基自由基、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基、超氧化物阴离子和羟基自由基的清除率分别为62.07%、68.50%、70.26%和75.52%。Xylo-oligosaccharides were prepared from Camellia oleifera shell via hydrothermal treatment after electron beam irradiation.The antioxidant activity of xylo-oligosaccharide of Camellia oleifera shell after decolorization was determined and analyzed through optimizing the decolorization process of activated carbon.This provides a new approach for the high-value utilization of by-products from Camellia oleifera processing.The results showed that the concentration of xylo-oligosaccharides prepared via 3 MeV electron beam irradiation combined with hydrothermal treatment was 13.87 mg/mL,and the concentrations of xylobiose to xylotetrose after enzymolysis were 3.37 mg/mL,4.76 mg/mL,and 2.48 mg/mL,respectively.The optimization process of activated carbon decolorization as follows:the decolorization efficiency of Darco G60-activated carbon for xylo-oligosaccharides enzymolysis solution of the Camellia oleifera shell reached 93.77%at a decolorization temperature of 30℃,a decolorization time of 60 min,an activated carbon addition of 50 mg/mL,and a pH of 4.The retention rates of xylobiose to xylotetrose and total xylo-oligosaccharides were 97.66%and 71.50%,respectively.It was found that the xylo-oligosaccharides of the Camellia oleifera shell were amorphous and cross-linked with several parts,as shown by scanning electron microscopy and Fourier transform infrared spectroscopy,with the main chain formed by Dxylose viaβ-1,4-glucoside linkage.In addition,when the concentration of xylo-oligosaccharides in Camellia oleifera shell was 10 mg/mL,the antioxidant activity was better.The scavenging rates of 1,1-Diphenyl-2-picrylhydrazyl(DPPH)radical,2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)radical,superoxide anion and hydroxyl radical were 62.07%,68.50%,70.26%and 75.52%,respectively.

关 键 词：辐照-水热处理 低聚木糖 活性炭 结构分析 抗氧化 

分 类 号：TS209[轻工技术与工程—食品科学] TL99[轻工技术与工程—食品科学与工程]