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作 者:彭宁 陈智仙 汪涛 李春阳 陈昭 张彦 PENG Ning;CHEN Zhixian;WANG Tao;LI Chunyang;CHEN Zhao;ZHANG Yan(National Key Laboratory of Agricultural Microbiology,Yichang 443003,China;Yichang Engineering and Technology Research Center of Nutrition and Health Food,Yichang 443003,China;The Hubei Provincial Key Laboratory of Yeast Function,Yichang 443003,China)
机构地区:[1]农业微生物资源发掘与利用全国重点实验室,湖北宜昌443003 [2]宜昌市营养健康食品工程技术研究中心,湖北宜昌443003 [3]酵母功能湖北省重点实验室,湖北宜昌443003
出 处:《中国酿造》2025年第4期80-87,共8页China Brewing
基 金:国家重点研发计划(2023YFF1104403)。
摘 要:为了探究人参粉发酵前后的成分变化及运动性疲劳的缓解作用,该研究分析发酵前后人参粉中总皂苷、稀有皂苷(Rg3、Rg3R、Rg5)、多糖、蛋白质、氨基酸及短链脂肪酸含量的变化,并构建小鼠实验模型验证其对运动性疲劳的缓解效果。结果表明,发酵后人参粉总皂苷含量提升44.4%,稀有皂苷Rg3、Rg3R、Rg5含量分别为人参粉发酵前的86倍、9.3倍和120倍;游离氨基酸的种类从16种增加至20种,总含量从4.04%提升至5.76%,乳酸含量从(35.89±3.59)mg/kg增加至(19702.20±1970.22)mg/kg。与模型对照组相比,发酵人参粉能明显延长小鼠力竭时间,降低小鼠血清中乳酸含量、丙二醛(MDA)、尿素氮(BUN)含量及肌酸激酶(CK)水平,增加肝脏糖原含量及乳酸脱氢酶(LDH)、抗氧化酶水平、三磷酸腺苷(ATP)酶活力,表明其有一定的缓解运动性疲劳的效果。In order to investigate the composition changes of ginseng powder before and after fermentation and the alleviating effect of exercise fatigue,the contents changes of total saponins,rare saponins(Rg3,Rg3R,Rg5),polysaccharide,protein,amino acid and short-chain faty acid in ginseng powder before and after fermentation were analyzed,and a mouse experimental model to verify the alleviating effect of ginseng powder on exercise fatigue was established.The results showed that the total saponins contents of ginseng powder after fermentation were increased by 44.4%,and the rare saponins Rg3,Rg3R and Rg5 contents were 86 times,9.3 times and 120 times of ginseng powder before fermentation,respectively.The types of free amino acids increased from 16 to 20,the total contents increased from 4.04%to 5.76%,and the lactic acid contents increased from(35.89±3.59)mg/kg to(19702.20+1970.22)mg/kg.Compared with the model control group,fermented ginseng powder could significantly prolong the exhaustive time of mice,reduce the lactic acid contents,malondialdehyde(MDA),urea nitrogen(BUN)and creatine kinase(CK)levels in serum of mice,increase the liver glycogen contents,lactate dehydrogenase(LDH),antioxidant enzyme levels,and adenosine triphosphate(ATP)enzyme activity,which showed that it had the effect of relieving exercise fatigue to some extent.
关 键 词:人参粉发酵 成分变化 稀有皂苷 小鼠模型 缓解运动性疲劳
分 类 号:TS201.1[轻工技术与工程—食品科学]
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