白玉菇优质高产菌株筛选试验  

Screening of High Quality and High Yield Strains of White Jade Mushroom

作　　者：武彬[1,2] 赵翔宇 王飞翔 苏敬红 李辛阳[5] 宫志远 辛淑荣 王前翔[3] 姚强 WU Bin;ZHAO Xiangyu;WANG Feixiang;SU Jinghong;LI Xinyang;GONG Zhiyuan;XIN Shurong;WANG Qianxiang;YAO Qiang(State Key Laboratory of Nutrient Use and Management/Shandong Key Laboratory of Agrioultural Non-Point Source Pollution Control/Institure of Agricultural Resources and Environment,Shandong Academy of Agruicultural Sciences,Jinan 250100,China;School of Biological Engineering,Shandong Polytechnics,Jinan 250104,China;Jining Hongxin Agricultural Technology Co.,LTD.,Jining 272113,China;Shandong Fuyou Fungus Industry Co.,LTD.,Jining 273501,China;Shandong Seven Rivers Biotechnology Co.,LTD.,Zibo 255100,China;Shandong Taima Biotechnology Co.,LTD.,Jining 273500,China;Chongqing Chengkou SongkunFungus-tech Development Co.,LTD,Chongqing 405900,China)

机构地区：[1]养分资源高效利用全国重点实验室/山东省农业面源污染防控重点实验室/山东省农业科学院农业资源与环境研究所,山东济南250100 [2]山东职业学院生物工程学院,山东济南250104 [3]济宁鸿鑫农业科技有限公司,山东济宁272113 [4]山东福友菌业股份有限公司,山东济宁273501 [5]山东七河生物科技股份有限公司,山东淄博255100 [6]山东泰马生物科技有限公司,山东济宁273500 [7]重庆市城口县松坤菌草科技开发有限责任公司,重庆405900

出　　处：《中国果菜》2025年第4期48-53,84,共7页China Fruit & Vegetable

基　　金：泰山产业领军人才工程(tscy20230640);山东省科技型中小企业创新能力提升工程(2022TSGC1264);山东省重点研发计划农业良种工程(2022LZGC023);山东省乡村振兴科技创新提振行动计划(2024TZXD013);重庆市技术创新与应用发展专项(CSTB2023TIAD-LDX0014);济南市农业科技攻关项目(GG202417)。

摘　　要：为获得白玉菇的优良菌株,提高白玉菇产量,利用甲基磺酸乙酯(EMS)诱变处理白玉菇菌株子实体,检测诱变后的菌株菌丝生长势、拮抗作用及总糖、PPO、CAT、MDA含量,筛选出菌丝生长快、保鲜能力强的新菌株。结果表明,在诱变后的菌株里筛选得到1株生长势较好、遗传稳定,且与原始菌株有拮抗作用的新菌株,保存24 d后,新菌株的PPO、MDA含量分别比原始菌株低3.00 U/mL和2.50μmol/g FW,CAT含量比原始菌株高80.00 U,总糖含量变化趋势比原始菌株更稳定,综合对比得出,新菌株表现更佳。通过栽培实验观察,与原始菌株相比,新菌株采收期几乎不开伞,菌丛较浓密,子实体生长整齐,比原始菌株节省了8.82%的生长时间,且单瓶质量21.9 g左右,综合表现优良,该试验结果可以为优良白玉菇的栽培提供理论依据。Excellent strains of white jade mushroom were obtained to provide support for increasing the yield of white jade mushroom.Ethanesulfonate(EMS)mutagenesis was used to treat the fruiting bodies of mushroom strains.By detecting the growth potential,antagonism and content of total sugar,PPO,CAT and MDA of the mutated strains,the new strains with fast growth and strong preservation ability were screened.The result showed a new strains with good growth potential,genetic stability and antagonism with the original strain was selected from the mutagenized strains.After 24 days of storage,the PPO and MDA contents of the new strain were 3.00 U/mL and 2.50μmol/g FW lower than those of the original strain,respectively,and the CAT contents were 80.00 U higher than those of the original strain.The variation trend of total sugar content was more stable than that of the original strain,and the new strain showed the best performance.Through cultivation experiment observation,compared with the original strain,the new strain hardly opened the umbrella during the harvesting period.The flora was dense and the fruiting body grows neatly,compared with the original strain,the growth time was saved by 8.82%.The weight of a single bottle was about 21.9 g,and the comprehensive performance was excellent.The experimental results could provide a choose for the cultivation of fine white jade mushroom.

关 键 词：白玉菇 品种筛选 甲基磺酸乙酯(EMS) 贮藏品质 

分 类 号：S646.7[农业科学—蔬菜学]