Duodenal mucosal ablation with irreversible electroporation reduces liver lipids in rats with non-alcoholic fatty liver disease  

作　　者：Jia-Wei Yu Qi Zhao Pei-Xi Li Ya-Xuan Zhang Bi-Xuan Gao Lin-Biao Xiang Xiao-Yu Liu Lei Wang Yi-Jie Sun Ze-Zhou Yang Yu-Jia Shi Yun-Fei Chen Meng-Bo Yu Hong-Ke Zhang Lei Zhang Qin-Hong Xu Lu Ren Dan Li Yi Lyu Feng-Gang Ren Qiang Lu 

机构地区：[1]National Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine,The First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061,Shaanxi Province,China [2]Department of Pediatric Surgery,The First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061,Shaanxi Province,China [3]Department of Abdominal Surgery,The First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061,Shaanxi Province,China [4]Department of International Medical Center,The First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061,Shaanxi Province,China [5]Department of Hepatobiliary Surgery,The First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061,Shaanxi Province,China

出　　处：《World Journal of Gastroenterology》2025年第16期89-100,共12页世界胃肠病学杂志(英文)

基　　金：Supported by the National Key Research and Development Program,No.2023YFF0713700 and No.2023YFF0713705;Common Technology R&D Platform of Shaanxi Province,No.2023GXJS-01-1-2;the Cyrus Tang Foundation Chung Ying Young Scholars Program.

摘　　要：BACKGROUND Duodenal mucosal ablation(DMA)using irreversible electroporation(IRE)with a glucagon-like peptide-1 receptor agonist has been clinically shown to reduce liver lipid deposition in non-alcoholic fatty liver disease(NAFLD).However,the specific metabolic contributions of DMA using IRE in NAFLD remain unclear.AIM To assess the feasibility and effectiveness of DMA using IRE in NAFLD rat models.METHODS Seven-week-old male Sprague-Dawley rats underwent DMA using IRE after 8 weeks on a high-fat diet.Two weeks post-treatment,duodenal and liver tissues and blood samples were collected.We evaluated differences in the duodenal wall structure,liver lipid deposition,enteroendocrine,claudin,and zonula ocludens-1 in the duodenal mucosa.RESULTS DMA using IRE could be safely performed in rats with NAFLD without duodenal bleeding,perforation,or stenosis.The duodenum healed well 2 weeks after DMA and was characterized by slimmer villi,narrower and shallower crypts,and thicker myenterons compared with the sham-control setting.Liver lipid deposition was reduced and serum lipid index parameters were considerably improved in the DMA setting.However,these improvements were independent of food intake and weight loss.In addition,enteroendocrine parameters,such as claudin,and zonula ocludens-1 levels in the duodenal mucosa,differed between the different settings in the DMA group.CONCLUSION By altering enteroendocrine and duodenal permeability,simple DMA using IRE ameliorated liver lipid deposition and improved serum lipid parameters in NAFLD rats.

关 键 词：Duodenal mucosal ablation Irreversible electroporation Non-alcoholic fatty liver disease ENTEROENDOCRINE Duodenal permeability 

分 类 号：R575.5[医药卫生—消化系统]