液相色谱-质谱/质谱法测定植物饮料中甜蜜素的不确定度评定  

Uncertainty evaluation in the determination of cyclamate in plant beverages by LC-MS/MS

作　　者：李璐涵 张娇娇 郭晓芳 程换玲 周晓月 张帆 许育民 LI Luhan;ZHANG Jiaojiao;GUO Xiaofang;CHENG Huanling;ZHOU Xiaoyue;ZHANG Fan;XU Yumin(Research Institute of Henan Food Industry Scientific Co.,Ltd.,Zhengzhou Henan 450000,China)

机构地区：[1]河南省食品工业科学研究所有限公司,河南郑州450000

出　　处：《河北省科学院学报》2025年第2期48-56,共9页Journal of The Hebei Academy of Sciences

基　　金：河南省重大科技专项项目(181100211400)。

摘　　要：本文采用GB 5009.97—2023规定中的第三法,以内标法来精准地检测植物饮料中的甜蜜素含量,同时结合相关的不确定性评估标准,详细地研究了各种可能的影响因素,以便更准确地获得结果。经分析计算得出,植物饮料中甜蜜素浓度为82.9 mg/kg,其浓度的扩展不确定度为4.8 mg/kg,由此得出甜蜜素检测结果为(82.9±4.8)mg/kg(P=95%,k=2)。结果表明,5个不确定度来源对该测量方法的影响从大到小依次为:标准溶液配制>标准曲线拟合>测量重复性>加标回收率>样品前处理。该研究为植物饮料中甜蜜素测量结果的准确性提供了科学依据,也是作为判断产品是否合格的重要依据。This study employed Method Ⅲ of GB 5009.97-2023 with internal standard method to quantify cyclamate concentrations in plant-based beverages.A comprehensive uncertainty evaluation was conducted to identify key influencing factors,so as to obtain more accurate results.Analysis and calculation demonstrated that the concentration of cyclamate in plant-based beverages was 82.9 mg/kg,and the expanded uncertainty of the concentration was 4.8 mg/kg,so the detection result of cyclamate was(82.9±4.8)mg/kg(P=95%,k=2).The results show that the influence of 5 uncertainty sources on the measurement method is as follows:standard solution preparation>standard curve fitting>measurement repeatability>spiked recovery rate>sample pretreatment.This study not only provides a scientific basis for the accuracy of the measurement results of cyclamate in plant-based beverages but also establishes an important criterion for determining product quality.

关 键 词：液相色谱-质谱/质谱法 植物饮料 甜蜜素 不确定度 

分 类 号：O657.6[理学—分析化学]