一株猪源肠出血性大肠杆菌O157:H7的全基因组测序及毒力和耐药基因分析  

Whole Genome Sequencing Reveals the Distribution of Resistance and Virulence Genes of an Enterohemorrhagic Escherichia coli O157∶H7 Strain from Swine

作　　者：周艳[1] 万启旸 朱树娇 张辉[1] 包红朵[1] 王冉[1] ZHOU Yan;WAN Qiyang;ZHU Shujiao;ZHANG Hui;BAO Hongduo;WANG Ran(Institute of Food Safety and Nutrition,Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base,Ministry of Science and Technology,Nanjing 210014,China)

机构地区：[1]江苏省农业科学院农产品质量安全与营养研究所/江苏省食品质量安全重点实验室-省部共建国家重点实验室培育基地,江苏南京210014

出　　处：《广东农业科学》2025年第2期58-70,共13页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(31972764)。

摘　　要：[目的]分析一株猪源肠出血性大肠杆菌O157:H7菌株EO157-1的全基因组信息及其毒力和耐药基因。[方法]基于Nanopore三代测序技术平台和Illumina二代测序技术平台对猪源肠出血性大肠杆菌O157:H7菌株EO157-1进行全基因组测序,利用Prokka软件预测基因组序列信息,并通过UniProt、KEGG、KEGG Pathway、GO、Pfam、COG、TIGERfams、RefSeq和NR数据库进行基因预测和注释,再利用碳水化合物酶(CAZy)、病原与宿主互作(PHI)、毒力因子(VFDB)和耐药基因(CARD)数据库进行基因功能分析。[结果]肠出血性大肠杆菌O157:H7菌株EO157-1基因组大小为5404747 bp,GC含量为50.49%,该菌株含有3个质粒;共预测到5674个蛋白质编码基因,包含22个rRNA(7个23S rRNA、7个16S rRNA、8个5S rRNA)、1个tmRNA、102个tRNA基因和282个小RNA分子;预测出9个CRISPR序列、273个重复序列;KEGG、KEGG Pathway、NR、UniProt、GO、COG、Pfam、RefSeq和TIGERfams数据库分别注释到1712、1693、5265、5264、4029、4294、4688、5252和2741个基因;CAZy数据库注释筛选出100个基因,PHI数据库注释筛选出2354个基因,VFDB数据库注释筛选出的毒力基因主要包括菌毛、鞭毛、Ⅱ型分泌系统、Ⅲ型分泌系统、Ⅵ型分泌系统、紧密黏附素、铁转运系统、脂多糖、荚膜、侵袭素和外毒素;CARD数据库注释筛选出的耐药基因与多重耐药外排泵、大环内酯类抗生素、四环素、多粘菌素、杆菌肽、氨基糖苷类抗生素、肽类抗生素及甲硝唑相关。[结论]获得一株肠出血性大肠杆菌O157:H7菌株EO157-1全基因组序列,序列分析表明该菌株携带大量毒力基因与耐药基因。【Objective】In order to analyze the whole genome information of a porcine derived enterohemorrhagic Escherichia coli O157∶H7 strain EO157-1 and its virulence and drug resistance genes.【Method】This experiment conducted whole-genome sequencing of enterohemorrhagic E.coli O157∶H7 strain EO157-1 based on the Nanopore third-generation sequencing technology platform and the Illumina second-generation sequencing technology platform.The Prokka software was used to predict the genome sequence information,and the genome sequence information was predicted through UniProt,KEGG and KEGG Pathway,GO,Pfam,COG,TIGERfams,RefSeq and NR databases were used for gene prediction and annotation,and then the carbohydrate enzyme(CAZy),pathogen and host interaction(PHI),virulence factor(VFDB)and drug resistance gene(CARD)databases were used Perform gene functional analysis.【Result】The genome size of enterohemorrhagic E.coli O157∶H7 strain EO157-1 is 5404747 bp,and the GC content is 50.49%.In addition,this strain also contains 3 plasmids.There are 5674 predicted protein-coding genes,including 22 rRNA(723S rRNA,716S rRNA,85S rRNA),1 tmRNA,102 tRNA genes and 282 small RNA molecules;9 are predicted CRISPR sequences,273 repeat sequences;KEGG,KEGG Pathway,NR,UniProt,GO,COG,Pfam,RefSeq and TIGERfams databases annotated 1712,1693,5265,5264,4029,4294,4688,5252 and 2741 genes respectively.The CAZy database annotation screened out 100 genes,the PHI database annotation screened out 2354 genes,and the VFDB database annotation screened out virulence genes mainly including pili,flagella,type II secretion system,type III secretion system,type VI secretion system,intimin,iron transport system,lipopolysaccharide,capsule,invasins and exotoxins;resistance genes and multidrug resistance efflux pumps,macrolide antibiotics,tetracyclines,polymyxins,bacitracin,and aminoglycoside antibiotics,peptide antibiotics and metronidazole related.【Conclusion】The whole genome sequence of an enterohemorrhagic E.coli O157∶H7 strain EO157-1 was obtained.Se

关 键 词：肠出血性大肠杆菌O157∶H7 食源性人兽共患病 全基因组测序 基因 功能注释 

分 类 号：S852[农业科学—基础兽医学]