产叶酸益生菌菌株筛选及发酵工艺优化  

Screening of Folic Acid Producing Probiotic Strains and Optimization of Fermentation Process

作　　者：徐心怡 毛鹏程 XU Xinyi;MAO Pengcheng(Yangzhou University,Yangzhou 225002,China)

机构地区：[1]扬州大学,江苏扬州225002

出　　处：《食品安全导刊》2025年第12期116-122,共7页China Food Safety Magazine

基　　金：扬州大学江苏省研究生实践创新计划资助项目“高产叶酸益生菌的筛选及功能特性研究”(SJCX23_1962)。

摘　　要：微生物发酵合成的叶酸具有良好的安全性,无明显副作用,且能被人体直接吸收利用。益生菌被认为是实现微生物合成叶酸的理想选择之一。叶酸产量高的菌株可用于开发营养价值更高的叶酸强化食品,具有良好的食品工业应用前景。本研究以从黄浆豆腐水、泡菜中分离纯化得到的益生菌为研究对象,以叶酸产量为主要评价指标,基于形态学特性和序列测定鉴别益生菌种类,通过去叶酸培养基对产叶酸益生菌进行初筛,并采用微生物法对其进行复筛,得到适合用于发酵合成叶酸的菌种,通过单因素试验和响应面试验设计优化发酵条件提高叶酸产量。结果表明,发酵乳杆菌50为产叶酸优势菌株,最佳发酵工艺条件为初始pH=6.5、发酵温度32℃、pABA添加量0.33 g·L^(-1)、葡萄糖为碳源、接种量2%和发酵时间20 h,在此条件下叶酸产量可达1.173 9μg·mL^(-1)。Folic acid synthesized by microbial fermentation has good safety,no obvious side effects,and can be directly absorbed and utilized by the human body.Probiotics are considered one of the ideal choices for microbial synthesis of folate.Strains with high folate production can be used to develop folate fortified foods with higher nutritional value,and have good prospects for application in the food industry.This study focuses on the isolation and purification of probiotics from Huangjiang Tofu water and kimchi,with folate production as the main evaluation indicator.Based on morphological characteristics and sequence determination,the types of probiotics are identified.The folate producing probiotics are initially screened using folate free culture medium,and then re screened using microbial methods to obtain strains suitable for fermentation synthesis of folate.The fermentation conditions are optimized through single factor experiments and response surface experiments to improve folate production.The results showed that Lactobacillus fermentum 50 was the dominant strain for producing folic acid.The optimal fermentation conditions were initial pH=6.5,fermentation temperature 32℃,pABA addition 0.33 g·L^(-1),glucose as the carbon source,inoculation amount 2%,and fermentation time 20 h.Under these conditions,the yield of folic acid could reach 1.1739μg·mL^(-1).

关 键 词：益生菌 叶酸 筛选 工艺优化 

分 类 号：TS201.3[轻工技术与工程—食品科学]