解淀粉芽孢杆菌的遗传操作与外源蛋白表达研究  

Genetic Manipulation and Exogenous Protein Expression of Bacillus amyloliquefaciens

作　　者：刘佳[1] 陈义杰 常菊花[1] LIU Jia;CHEN Yijie;CHANG Juhua(College of Life Science,Yangtze University,Jingzhou 434025,China;College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]长江大学生命科学学院,湖北荆州434025 [2]华中农业大学食品科技学院,湖北武汉430070

出　　处：《食品安全导刊》2025年第12期176-179,共4页China Food Safety Magazine

摘　　要：解淀粉芽孢杆菌是一种广泛分布于植物、土壤及海水等自然环境中的革兰氏阳性细菌。由于其具有高效的蛋白分泌能力,该菌株已成为极具应用潜力的外源蛋白表达宿主。本文系统综述了解淀粉芽孢杆菌的遗传操作体系(包括遗传转化方法和表达载体),分析了外源蛋白表达及影响蛋白表达关键因素,旨在为解淀粉芽孢杆菌的有效利用提供理论依据。Bacillus amyloliquefaciens is a Gram positive bacterium widely distributed in natural environments such as plants,soil,and seawater.Due to its efficient protein secretion ability,this strain has become a highly promising host for exogenous protein expression.This article provides a systematic review of the genetic manipulation system of Bacillus amyloliquefaciens,including genetic transformation methods and expression vectors.It analyzes the expression of exogenous proteins and key factors affecting protein expression,aiming to provide theoretical basis for the effective utilization of Bacillus amyloliquefaciens.

关 键 词：解淀粉芽孢杆菌 遗传操作体系 外源蛋白表达 影响因素 

分 类 号：Q78[生物学—分子生物学]