巨噬细胞吞噬实验跨学科混合式教学模式的探索与实践  

Exploration and Practice of Interdisciplinary Mixed Teaching Mode of Macrophage Phagocytosis Experiment

作　　者：李奇志[1] 张耀文 袁雅燕 刘亚丰[1] 周爱文[1] 阮晶 雷敏 LI Qizhi;ZHANG Yaowen;YUAN Yayan;LIU Yafeng;ZHOU Aiwen;RUAN Jing;LEI Min(College of Life Sciences,Huazhong University of Science and Technology,Wuhan 430074,China)

机构地区：[1]华中科技大学生命科学与技术学院,武汉430074

出　　处：《中国细胞生物学学报》2025年第4期867-879,共13页Chinese Journal of Cell Biology

基　　金：华中科技大学实验技术研究项目(批准号:HZKJSYJSXM2024M024)资助的课题。

摘　　要：吞噬实验是细胞生物学、免疫学和微生物学的经典实验,但当前的实验教学在内容设计上既缺乏学科之间的融合,又鲜有把虚拟实验应用在吞噬实验教学中。笔者所在教学团队以小鼠巨噬细胞系RAW264.7为实验对象,荧光标记的大肠杆菌BL21(pET-28a-mCherry)为示踪物,探讨巨噬细胞经典活化前后细胞形态与吞噬功能的改变。根据授课学生的知识领域特点,设计以细胞生物学为主线内容,免疫学和微生物学等学科作为辅助和补充,借助线上学习平台和学院优质的虚拟实验平台,为学生提供与线下教学紧密联系的线上学习内容。同时采用传统经典课堂教学结合半开放实验教学的多元化混合式教学模式,并建立多维的考核评价体系。通过这些教学改革举措的实施,把与吞噬作用相关的细胞生物学、免疫学和微生物学等学科知识点有机地融合在一起,旨在使学生能够更深入全面地理解吞噬作用这一生命活动,有效地激发了学生创新和探索的意识,同时也拓展了实验教学模式。Phagocytosis experiment is a classic experiment of cell biology,immunology and microbiology.However,the current experimental teaching rarely considers the integration of these disciplines in content design,and there are few applications of virtual experiments in the teaching of phagocytic experiments.The teaching team of the author introduced mouse macrophage line RAW264.7 as the experimental object and fluorescent labeled Escherichia coli BL21(pET-28a-mCherry)as the tracer to explore the changes of cell morphology and phagocytic function before and after classical activation of macrophages.According to the characteristics of the knowledge field of the students,the content of cell biology is designed as the main line,and the disciplines of immunology and microbiology are used as auxiliary and supplement.With the help of online learning platform and the college's high-quality virtual experiment platform,online learning content closely related to offline teaching is provided for students;at the same time,this team adopted the diversified mixed teaching mode combined traditional classical classroom teaching with semi-open experimental teaching,and established a multi-dimensional evaluation system.Through the implementation of these teaching reform measures,the knowledge points of cell biology,immunology,microbiology and other disciplines related to phagocytosis are organically integrated,aiming to enable students to comprehensively understand phagocytosis,which is a life activity,effectively stimulate students'awareness of innovation and exploration,and expand the experimental teaching mode.

关 键 词：细胞生物学实验 教学改革 巨噬细胞RAW264.7 吞噬作用 混合式教学 

分 类 号：G642[文化科学—高等教育学]