白藜芦醇微胶囊的工艺优化及其对人胃癌细胞的抑制作用  

Process optimization of resveratrol microcapsule and the inhibitory effect on human gastric cancer cells

作　　者：张乐乐 姜璐晴 张滕 乔勤勤 刘生杰 兰伟 ZHANG Lele;JIANG Luqing;ZHANG Teng;QIAO Qinqin;LIU Shengjie;LAN Wei(School of Biology and Food Engineering,Fuyang Normal University,Fuyang 236037,China;College of Information Engineering,Fuyang Normal University,Fuyang 236041,China;School of Biology and Food Engineering,Fuyang University of Technology,Fuyang 236000,China;Anhui Ecological Fermentation Engineering Research Center)

机构地区：[1]阜阳师范大学生物与食品工程学院,阜阳236037 [2]阜阳师范大学信息工程学院食品系,阜阳236041 [3]阜阳理工学院生物与食品工程学院,阜阳236000 [4]安徽省功能性果饮生态发酵工程研究中心,阜阳236037

出　　处：《武汉轻工大学学报》2025年第2期93-101,共9页Journal of Wuhan Polytechnic University

基　　金：安徽省高校科研重点项目(编号:2023AH052852、2024AH051448、2024AH051449);安徽省大学生创新训练项目(编号:s202313619006).

摘　　要：利用β-环糊精包埋白藜芦醇制备微胶囊,以提高白藜芦醇的生物利用度并探究其对人胃癌细胞的抑制作用。对白藜芦醇微胶囊进行工艺优化以提高其水溶性,基于单因素试验结果,结合响应面试验结果,得到白藜芦醇微胶囊的最佳工艺条件为:白藜芦醇与β-环糊精质量比为1∶3,搅拌时间为36 min,搅拌温度为42℃。在此工艺条件下白藜芦醇微胶囊载的药量为(19.15±0.46)%。白藜芦醇浓度为0.75μg/mL的微胶囊作用人胃癌细胞HGC-2724 h之后的细胞存活率是(50.39±1.58)%。白藜芦醇微胶囊有望在人胃癌治疗的过程中发挥一定作用。β-cyclodextrin was utilized to encapsulate resveratrol,prepare microcapsules to enhance the bioavailability of resveratrol and investigate its inhibitory effects on human gastric cancer cells.Based on the results of single-factor test and combined with the results of response surface method,the optimal process conditions for resveratrol microcapsules were as follows:the mass ratio of resveratrol beta-cyclodextrin was 1∶3,the mixing time was 36 min,and the stirring temperature was 42℃.Under these process conditions,the drug loading of resveratrol microcapsules was(19.15±0.46)%.The cell survival rate of HCG-27 human gastric cancer cells treated with microcapsules with a resveratrol concentration of 0.75μg/mL for 24 hours was(50.39±1.58)%.Therefore,resveratrol microcapsules are expected to play a certain role in the treatment of human gastric cancer.

关 键 词：白藜芦醇 Β-环糊精 微胶囊 人胃癌细胞HGC-27 

分 类 号：TQ464[化学工程—制药化工]