上海市浦东新区市售食品中创伤弧菌病原学遗传特征  

Pathogenic genetic characteristics of Vibrio vulnificus in commercial foods in Pudong New Area of Shanghai

作　　者：梁艳茹 吕彬 胡旖桓 丁锦 桂燕华 邱慧芳[1,2] 丰俊 赵冰 王筱[1,2] LIANG Yanru;LYU Bin;HU Yihuan;DING Jin;GUI Yanhua;QIU Huifang;FENG Jun;ZHAO Bing;WAN Xiao(Shanghai Pudong New Area Center for Disease Control and Prevention,Shanghai 200136,China;Fudan University Pudong Institute of Preventive Medicine,Shanghai 200136,China;Shanghai Municipal Center for Disease Control and Prevention,Shanghai 200336,China)

机构地区：[1]上海市浦东新区疾病预防控制中心,上海200136 [2]复旦大学浦东预防医学研究院,上海200136 [3]上海市疾病预防控制中心,上海200336

出　　处：《中国热带医学》2025年第1期87-94,共8页China Tropical Medicine

基　　金：上海市加强公共卫生体系建设三年行动计划(2023-2025年)重点学科(GWVI-11.1-02-传染病学);上海市浦东新区疾病预防控制中心科研项目(No.PDCDC-2023-09)。

摘　　要：目的了解上海市浦东新区市售食品中创伤弧菌污染情况、耐药基因和毒力基因携带情况、基因分型特征与遗传进化关系。方法采用微量肉汤法进行药物敏感性实验,使用illumina测序平台进行全基因组测序,基于毒力基因数据库、耐药基因数据库、PubMLST数据库等预测毒力基因、耐药基因及多位点序列分型(multilocus sequence typing,MLST),基于全基因组单核苷酸多态性(single nucleotide polymorphism,SNP)构建系统发育树进化分析。结果2023年市售食品中创伤弧菌检出率为4.56%(51/1119),优势检出类别依次为贝类(20.16%,25/124)、鱼类(6.74%,19/282)、虾类(6.56%,4/61)、即食水产类(9.09%,2/22)及畜肉类(0.33%,1/300)。创伤弧菌对头孢唑啉耐药率最高为23.50%(12/51),其次是氨苄西林、四环素和复方新诺明。携带四环素类(tetA、tetC)、喹诺酮类(QnrVC6、QnrVC4)、碳青霉烯类(varG)等29种耐药基因,鞭毛(fla E)、铁离子吸收(hutA)等相关毒力基因163种。MLST结果共发现41种ST型别(1株未分型),其中新产生ST型别32种,有38株分离株为新ST型,优势ST型别为ST326(5.88%,3/51)和ST667(5.88%,3/51)。SNP结果显示所有菌株分为3大分支,系统发育树内部分支较长,具有较高的同源重组性。结论上海市浦东新区市售食品中,贝类和鱼类中创伤弧菌污染严重。分离的菌株耐药谱广泛,存在多重耐药现象,且携带多种毒力及耐药相关基因。菌株基因组高度同源重组,具有一定的食品安全隐患风险,建议加强对其监测管理。Objective To understand the contamination status,drug resistance,virulence,genotyping characteristics,and genetic evolution relationships of Vibrio vulnificus in commercially available food in Pudong New Area of Shanghai.Methods The drug susceptibility test was carried out by microbroth dilution method.The whole genome sequencing was used by illumina sequencing platform.The virulence,drug resistance genes and MLST typing were obtained by VFDB,CARD and PubMLST database.Phylogenetic analysis was used by genome-wide single nucleotide polymorphisms.Results The detection rate of Vibrio vulnusis in commercially available food was 4.56%(51/1119)in 2023,and the dominant detection categories were shellfish(20.16%,25/124),fish(6.74%,19/282),shrimp(6.56%,4/61),ready-to-eat aquatic products(9.09%,2/22)and animal meat(0.33%,1/300).The highest drug resistance rate of Vibrio vulnificus to cefazolin is 23.5%(12/51),followed by ampicillin,tetracycline,and trimethoprim sulfamethoxazole.And 51 strains of Vibrio vulnificus carried 29 kinds of drug resistance genes such as tetracycline(tetA,tetC),quinolones(QnrVC6,QnrVC4),carbapenems(varG),and 163 kinds of virulence genes related to flagella(fla E)and iron ion absorption(hutA).The results of MLST analysis showed that there were 41 ST types(one untyped),32 new ST types were generated,and 38 isolates were new ST types,mainly ST326(5.88%,3/51)and ST667(5.88%,3/51).SNP showed that all strains were divided into three branches,and the branches in the phylogenetic tree were longer,and had higher homologous recombination.Conclusion In Pudong New Area,the contamination of Vibrio vulnificus was serious in shellfish,fish,shrimp,ready-to-eat aquatic products and animal meat.The drug resistance spectrum is wide and there are multiple drug resistance phenomena.It carries a variety of virulence genes and drug-resistant genes,and is highly homologous recombination,which has certain food safety risks,and should be strengthened monitoring and management.

关 键 词：创伤弧菌 耐药 毒力基因 耐药基因 系统进化分析 市售食品 

分 类 号：R561[医药卫生—呼吸系统]