广州市2023年13株登革病毒2型全基因组序列特征分析  

Whole genome sequence characteristics of 13 dengue virus serotype 2 strains isolated from Guangzhou in 2023

作　　者：马晋哲 梁玉凤 周志坚 冯晓雯 李芬香 陈绮 严华成 万成松[1] MA Jinzhe;LIANG Yufeng;ZHOU Zhijian;FENG Xiaowen;LI Fenxiang;CHEN Qi;YAN Huacheng;WAN Chengsong(School of Public Health,Southern Medical University,Guangzhou,Guangdong 510515,China;Center for Disease Control and Prevention of Southern Theatre Command,Guangzhou,Guangdong 510507,China)

机构地区：[1]南方医科大学公共卫生学院,广东广州510515 [2]南部战区疾病预防控制中心,广东广州510507

出　　处：《中国热带医学》2025年第1期110-116,共7页China Tropical Medicine

摘　　要：目的分析2023年广州市登革病毒2型(dengue serotype 2 virus,DENV-2)分离株的全基因组序列特征,为本地登革热防控提供新的基础数据。方法使用C6/36细胞分离登革热患者血清中的登革病毒,使用纳米孔测序技术(Nanopore平台)对登革病毒进行全基因组测序,使用IPH-NANO v1.0软件对测序数据进行组装,使用BioEdit 7.0.9.0、MEGA 11、iqTree 1.6.12等软件对登革病毒基因组序列进行同源性分析、系统进化分析及氨基酸位点差异分析。结果从2023年广州市登革热患者的血清样本中分离得到13株DENV-2分离株(均来自本地感染病例);测序后拼接获得的全基因组序列长度范围为10429~10439 nt;13株分离株间的核苷酸(氨基酸)同源性为99.7%~99.9%(99.6%~99.9%);13株广州分离株的基因型均为Cosmopolitan型,且与法属留尼汪、吉布提、肯尼亚等地的分离株处于同一进化枝;与参考序列(NC001474)相比,13株分离株的C/prM/E蛋白区共有26个氨基酸位点出现差异,其中C蛋白区域有4个位点出现差异,prM蛋白区域有8个位点出现差异,E蛋白区域有14个位点出现差异。结论2023年广州市DENV-2分离株之间高度同源,与其亲缘关系较近的分离株主要来自法属留尼汪、吉布提、肯尼亚等国家(地区),提示需进一步研判源自东非、南亚等地区的登革热输入风险。Objective To analyze the whole genome sequence characteristics of dengue serotype 2 virus(DENV-2)strains isolated from Guangzhou in 2023,providing new foundational data to support local dengue fever prevention and control efforts.Methods Dengue viruses were isolated from serum samples of dengue fever patients using C6/36 cells.Whole genome sequencing of dengue virus was performed using Nanopore sequencing technology(Nanopore platform).The sequencing data were assembled utilizing IPH-NANO v1.0 software,and homology analysis,phylogenetic analysis,and amino acid site variation analysis of the dengue virus genome sequence were conducted using software such as BioEdit7.0.9.0,MEGA11,and iqTree1.6.12.Results A total of 13 DENV-2 isolates were obtained from the serum samples of dengue fever patients in Guangzhou in 2023,all of which were derived from locally infected cases.The whole genome sequences obtained by sequencing and splicing ranged from 10429-10439 nt in length.The nucleotide(amino acid)homology among the 13 isolates was 99.7%-99.9%(99.6%-9.9%).A genome-wide phylogenetic analysis showed that the genotypes of all 13 Guangzhou isolates were identified as the Cosmopolitan genotype and were grouped in the same evolutionary clade as isolates from Réunion(French overseas territory),Djibouti,Kenya,and other regions.Compared with the reference sequence(NC001474),a total of 26 amino acid site variations were identified in the C/prM/E protein regions of the 13 isolates,including 4 variations in the C protein region,8 in the prM protein region,and 14 in the E protein region.Conclusion The DENV-2 strains isolated from Guangzhou in 2023 exhibited high homology,with closely related isolates primarily originating from countries or regions such as Réunion,Djibouti,and Kenya,it highlights the need for further assessment of the risk of dengue fever importation from East Africa,South Asia,and other regions.

关 键 词：登革热 登革病毒2型 全基因组测序 纳米孔测序技术 系统进化分析 广州 

分 类 号：R373.33[医药卫生—病原生物学]