桑叶蛋白超声辅助提取新工艺优化及其抗氧化活性研究  

Optimization of New Ultrasound-Assisted Extraction Process for Mulberry Leaf Protein and Investigation of Its Antioxidant Activity

作　　者：周一晨 卫旭彪[1] 李震震 张皓森 张日俊[1] ZHOU Yichen;WEI Xubiao;LI Zhenzhen;ZHANG Haosen;ZHANG Rijun(State Key Laboratory of Animal Nutrition,Laboratory of Feed Biotechnology,China Agricultural University,Beijing 100193,China)

机构地区：[1]中国农业大学饲料生物技术实验室,动物营养学国家重点实验室,北京100193

出　　处：《动物营养学报》2025年第4期2716-2727,共12页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：十四五国家重点研发计划项目(No.2022YFD1300900)。

摘　　要：本试验旨在优化桑叶蛋白的提取工艺并探究桑叶蛋白的体外抗氧化活性。试验在利用超声破碎辅助碱溶酸沉法提取桑叶蛋白的基础上,先进行单因素试验,再结合单因素试验结果,采用Box-Behnken响应面法对料液比、浸提温度、超声时间和超声功率4个因素进一步优化,以确定最佳的提取条件;并进一步通过化学抗氧化能力试验测定新工艺提取的桑叶蛋白的生物活性。结果表明:1)经优化后得到桑叶蛋白提取的最佳工艺为:料液比1∶24(g/mL)、浸提温度30℃、超声时间4.9 min、超声功率235 W、浸提液pH 11、浸提时间60 min,此条件下桑叶蛋白的提取率为34.12%。2)提取的桑叶蛋白在0.4 mg/mL的浓度下对2,2′-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基、1,1-二苯基-2-三硝基苯肼(DPPH)和羟基自由基的体外清除率分别达到100.47%、74.92%和34.94%,还原力达到0.43。综上可知,本试验通过单因素试验和响应面试验优化了桑叶蛋白的提取工艺,并且在此工艺下提取的桑叶蛋白表现出较好的抗氧化活性。This study was conducted to optimize the extraction process of mulberry leaf protein and explore its antioxidant activity in vitro.On the basis of the extraction of mulberry leaf protein by ultrasonic crushing assisted alkali-solution and acid-isolation method,a single factor test was carried out first,and then combined with the results of single factor test,the Box-Behnken response surface method was used to further optimize the solid-liquid ratio,leaching temperature,ultrasonic time and ultrasonic power,so as to determine the best extraction conditions.Then the biological activity of mulberry leaf protein extracted by the new process was determined by chemical antioxidant capacity assay.The results showed as follows:1)the optimized extraction process of mulberry leaf protein was as follows:solid-liquid ratio of 1∶24(g/mL),leaching temperature 30℃,ultrasonic time 4.9 min,ultrasonic power 235 W,pH 11 in leaching solution,leaching time 60 min.Under these conditions,the extraction rate of mulberry leaf protein was 34.12%.2)At the concentration of 0.4 mg/mL,the scavenging rates of 2,2-biazo-bis(3-ethyl-benzothiazole-6-sulfonic acid)diammonium salt(ABTS)radical,1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)radical and hydroxyl radical reached 100.47%,74.92%and 34.94%,respectively,and the reducing power reached 0.43.In conclusion,this study optimized the extraction process of mulberry leaf protein through single factor test and response surface test,and the extracted mulberry leaf protein showed better antioxidant activity under this process.

关 键 词：桑叶蛋白 碱溶酸沉法 超声破碎 工艺优化 抗氧化活性 

分 类 号：S816[农业科学—饲料科学]