Grafts of hydrogel-embedded electrically stimulated subventricular stem cells into the stroke cavity improves functional recovery of mice  

作　　者：Andreea-Mihaela Cercel Ianis KS Boboc Roxana Surugiu Thorsten R.Doeppner Dirk M.Hermann Bogdan Catalin Andrei Gresita Aurel Popa-Wagner 

机构地区：[1]Experimental Research Center for Normal and Pathological Aging,University of Medicine and Pharmacy Craiova,Craiova,Romania [2]Doctoral School,University of Medicine and Pharmacy Craiova,Craiova,Romania [3]Department of Clinical Pharmacy,University of Medicine and Pharmacy of Craiova,Craiova,Romania [4]Department of Neurology,University Medical Center Göttingen,Göttingen,Germany [5]Chair of Vascular Neurology and Dementia,Department of Neurology,University Hospital Essen,Essen,Germany [6]Department of Neurology,University of Giessen Medical School,Giessen,Germany [7]Department of Biomedical Sciences,New York Institute of Technology,College of Osteopathic Medicine,Old Westbury,NY,USA

出　　处：《Neural Regeneration Research》2026年第2期695-703,共9页中国神经再生研究(英文版)

基　　金：supported by European Union Funding Programme,PNRR,No.760058(to DMH)and the UEFISCDI Project,No.PN-III-P4-ID-PCE-2020-059(to APW).#。

摘　　要：The major aim of stroke therapy is to stimulate brain repair and improve behavioral recovery after cerebral ischemia.One option is to stimulate endogenous neurogenesis in the subventricular zone and direct the newly formed neurons to the damaged area.However,only a small percentage of these neurons survive,and many do not reach the damaged area,possibly because the corpus callosum impedes the migration of subventricular zone-derived stem cells into the lesioned cortex.A second major obstacle to stem cell therapy is the strong inflammatory reaction induced by cerebral ischemia,whereby the associated phagocytic activity of brain macrophages removes both therapeutic cells and/or cell-based drug carriers.To address these issues,neurogenesis was electrically stimulated in the subventricular zone,followed by isolation of proliferating cells,including newly formed neurons,which were subsequently mixed with a nutritional hydrogel.This mixture was then transferred to the stroke cavity of day 14 post-stroke mice.We found that the performance of the treated animals improved in behavioral tests,including novel object,open field,hole board,grooming,and“time-to-feel”adhesive tape tests.Furthermore,immunostaining revealed that the stem cell marker nestin,the neuroepithelial marker Mash1,and the immature neuronal marker doublecortin-positive cells survived in the transplanted area for 2 weeks,possibly due to reduced phagocytic activity and supportive angiogenesis.These results clearly indicate that the transplantation of committed subventricular zone stem cells combined with a protective nutritional gel directly into the infarct cavity after the peak of stroke-induced neuroinflammation represents a feasible approach to improve neurorestoration after cerebral ischemia.

关 键 词：ANXA3 behavioral recovery DOUBLECORTIN electrical stimulation Mash1 NESTIN STROKE subventricular neural stem cells supportive hydrogel vascular cell adhesion molecule 1 

分 类 号：R743.3[医药卫生—神经病学与精神病学]