A single-cell landscape of the regenerating spinal cord of zebrafish  

作　　者：Lei Yao Xinyi Cai Saishuai Yang Yixing Song Lingyan Xing Guicai Li Zhiming Cui Jiajia Chen 

机构地区：[1]Department of Anesthesiology,Affiliated Hospital 2 of Nantong University,Nantong,Jiangsu Province,China [2]Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education,Co-Innovation Center of Neuroregeneration,NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products,Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair,Nantong University,Nantong,Jiangsu Province,China [3]Department of Spine Surgery,Affiliated Hospital 2 of Nantong University,Nantong,Jiangsu Province,China [4]Research Institute for Spine and Spinal Cord Disease of Nantong University,Nantong,Jiangsu Province,China

出　　处：《Neural Regeneration Research》2026年第2期780-789,共10页中国神经再生研究(英文版)

基　　金：supported by the Jiangsu Province Traditional Chinese Medicine Technology Development Plan Project,Nos.MS2023113(to JC),MS2022090;Young and Middle-aged Academic Leaders of Jiangsu Qing-Lan Project(to GL).

摘　　要：Unlike mammals,zebrafish possess a remarkable ability to regenerate their spinal cord after injury,making them an ideal vertebrate model for studying regeneration.While previous research has identified key cell types involved in this process,the underlying molecular and cellular mechanisms remain largely unexplored.In this study,we used single-cell RNA sequencing to profile distinct cell populations at different stages of spinal cord injury in zebrafish.Our analysis revealed that multiple subpopulations of neurons showed persistent activation of genes associated with axonal regeneration post injury,while molecular signals promoting growth cone collapse were inhibited.Radial glial cells exhibited significant proliferation and differentiation potential post injury,indicating their intrinsic roles in promoting neurogenesis and axonal regeneration,respectively.Additionally,we found that inflammatory factors rapidly decreased in the early stages following spinal cord injury,creating a microenvironment permissive for tissue repair and regeneration.Furthermore,oligodendrocytes lost maturity markers while exhibiting increased proliferation following injury.These findings demonstrated that the rapid and orderly regulation of inflammation,as well as the efficient proliferation and redifferentiation of new neurons and glial cells,enabled zebrafish to reconstruct the spinal cord.This research provides new insights into the cellular transitions and molecular programs that drive spinal cord regeneration,offering promising avenues for future research and therapeutic strategies.

关 键 词：dividing oligodendrocyte macrophage MICROGLIA neuron proliferating oligodendrocyte radial glia single cell sequencing spinal cord regeneration transcriptome ZEBRAFISH 

分 类 号：R651.2[医药卫生—外科学]