毕赤酵母表达系统研究进展  

Advances in Pichia Expression System

作　　者：齐连权[1] 陈薇[1] 来大志[1] 于长明[1] 王海涛[1] 

机构地区：[1]军事医学科学院微生物学流行病学研究所,北京100071

出　　处：《中国生物工程杂志》2002年第6期45-47,共3页China Biotechnology

摘　　要：毕赤酵母表达系统是目前最为成功的外源蛋白表达系统之一。该表达系统不存在原核表达系统的内毒素难以除去的问题 ,也不存在哺乳动物细胞表达系统的病毒和支原体污染等问题 ;并能够对目的蛋白进行类似高等真核生物的信号肽剪切、二硫键形成、糖基化等过程的翻译后蛋白加工。至今已有多种外源蛋白在该表达系统中成功表达。下面对毕赤酵母表达系统的特点及研究进展作一简要综述。Pichia expression system is one of the most successful foreign protein expression system until now.It does not have the endotoxin problem associated with bacteria nor the viral contamination problem of proteins produced in animal cell culture; Pichia pastoris has the potential to perform many of the posttranslational modifications typically associated with higher eukaryotes.These include processing of signal sequence processing,disulfide bridge formation and glycosylation.To date Many heterologous proteins have been expressed in Pichia pastoris successfully.In this review,the recent advances of the system are described and discussed.\;

关 键 词：毕赤酵母 表达系统 研究进展 

分 类 号：Q786[生物学—分子生物学]