γ-干扰素基因修饰A549细胞瘤苗研究  

STUDY ON VACCINATION OF A549 CELL LINES TRANSDUCED WITH HUMAN IF-γ GENE

作　　者：于晓虹[1] 刘祥麟[1] 马洁羽[1] 

机构地区：[1]浙江大学医学院肿瘤基因研究组,杭州310031

出　　处：《细胞生物学杂志》2002年第6期379-382,共4页Chinese Journal of Cell Biology

基　　金：杭州市科委(99113A07);杭州市卫生局(招9801)

摘　　要：本文研究了将人IFN-γ基因重组入pLXSN 载体,用pA317包装后转入人肺腺癌细胞株A549中,经G418筛选获得稳定表达IFN-γ的转基因的A549-IFN-γ细胞株。经测定,该转基因的细胞表面MHC-Ⅰ、Ⅱ分子表达显著提高。接种动物表明致瘤性下降,制成瘤苗可诱导产生较强的细胞毒T淋巴细胞的抑瘤能力;经放射线照射的瘤苗,在添加肿瘤特异抗原和细胞因子后,在动物肿瘤模型上显示出良好的抑瘤效果。提示,经IFN-γ基因转导的人肺腺癌A549细胞株可产生较强的抗肿瘤效果,这为该瘤苗的临床应用提供了实验依据。We transduced the human interferon-γ(IFN-γ)gene into A549 cells,a human lung adenocarcinoma cell line,using revroviral vector pLXSN as a gene transfer vehicle. A high level of IFN-γ was secreted by IFN-γ gene-transduced A549 cells (A549-IFN-γ). The cells showed increased MHC- Ⅰ ,Ⅱ molecule expression after IFN-γ gene transfer. The gene-modified tumor cells had a very lower tumorigen-esis than its parental tumor cells. The spleen cells of mice were transplanted with A549-IFN-γ cells exhibited high cytotoxic activity compared to those from mice were transplanted with A549 cells and from untreated mice. The vaccine was irradiated by γ-ray radiation, and then immunialized mice. Five out of six mice were treated with the transgeneic without tumor appearance. These results indicated that the human IFN-γ gene-transduced lung cancer cells is a promising straegy for inducing antitumor immunity in the treatment of lung cancer.

关 键 词：肿瘤疫苗 转基因疫苗 肿瘤生物治疗 γ-干扰素基因修饰 A549细胞瘤苗 

分 类 号：R73-3[医药卫生—肿瘤]