粗糙脉孢菌(Neurospora crassa)木聚糖酶的合成及性质研究  

Production and Some Properties of Xylanase from Neurospora crassa

作　　者：张潇[1] 朱冬青[1] 王丹[1] 林建强[1] 曲音波[1] 余世袁[2] 

机构地区：[1]山东大学微生物技术国家重点实验室,济南250100 [2]南京林业大学化工学院,南京210037

出　　处：《纤维素科学与技术》2002年第4期20-26,共7页Journal of Cellulose Science and Technology

基　　金：山东省自然科学基金资助项目(编号:Y2001D11)

摘　　要：粗糙脉孢菌1602(Neurosporacrassa)可以产生木聚糖酶。以不同的植物纤维物料及纯木聚糖作为底物都可诱导产生较高的木聚糖酶活,其中玉米芯粉的诱导能力最强,酶活可达25.02IU/mL。纤维素与木聚糖混合对木聚糖酶合成具有促进作用。葡萄糖、木糖对木聚糖酶的合成有很强的阻遏作用,葡萄糖的阻遏作用大于木糖的阻遏作用。其木聚糖酶最适作用pH值为5.4,最适作用温度为60℃。Neurospora crassa As 3.1602 could produce xylanase using various kinds of cellulosic materials as well as pure xylan as the substrates and inducers. Xylase production reaches as high as 25.02 IU/mL using corn core powder, which is the strongest inducer tested. The mixture of cellulose and xylan increases xylase production compared with cellulose or xylose only. Glucose and xylose repress xylase production strongly, and the repression effect of glucose is stronger than xylose. The optimal pH and temperature of the xylase are 5.4 and 60℃, respectively.

关 键 词：粗糙脉孢菌 木聚糖酶 阻遏 

分 类 号：Q936[生物学—微生物学]