麦白霉素发酵培养基的改进  

The improvement of medium composition for meleumycinum fermentation

作　　者：王晓磊[1] 刘建周[1] 胡卫新[1] 李小兵[1] 薛可轶[1] 

机构地区：[1]中国矿业大学化工学院,江苏徐州221008

出　　处：《工业微生物》2002年第4期32-35,共4页Industrial Microbiology

摘　　要：研究了淀粉水解液全部替代葡萄糖作为碳源发酵麦白霉素的工艺条件。用正交实验得出摇瓶培养基消毒后的最佳基质浓度为总糖 5 .0g 10 0mL ,还原糖 3.8g 10 0mL ,氨基氮 60mg mL ,溶解磷 30 0 μg mL。以 5 0L发酵罐进行实验 ,得出了麦白霉素的发酵代谢曲线和淀粉的适宜水解条件。经 30t发酵罐放大实验结果表明 ,与葡萄糖作碳源相比 ,用淀粉水解液作碳源发酵麦白霉素 ,发酵单位提高 2 3.0 % ,生产成本降低 38.0 %。Fermentation conditions of meleumycinum using starch digest as carbon source instead of glucose were studied. Based on shake flask experiments with orthogonal experiment design method the optimum substratum concentration were as follows: the concentration of total sugar was 5.0g/100mL, the reduced sugar was 3.8g/100mL, the amino-nitrogen was 60mg/mL, and the lytic-phosphorus was 300μg/mL. The fermentation metabolic curve and suitable hydrolysis conditions were obtained by experiments carried in 50L fermentor. The results of pilot experiments on scale of 30t fermentor indicated that, compared with glucose as carbon source for meleumycinum fermentation, the fermentation unity increased by 23.0% and the production cost decreased by 38.0%, when starch digest was adopted as carbon source.

关 键 词：麦白霉素 发酵 培养基 改进 淀粉水解液 碳源 

分 类 号：TQ465[化学工程—制药化工]