联用重组生长激素、膳食纤维促短肠大鼠结肠形态代偿  

To Promote Colonic Morphological Adaption of Short Bowel Rat by Combination of Enteral Nutrition, Diet Fiber and Recombinant Growth Hormone

作　　者：许剑民[1] 何连齐[1] 吴新华[1] 靳大勇[1] 吴海福[1] 吴肇汉[1] 

机构地区：[1]复旦大学附属中山医院普外科,上海200032

出　　处：《中国临床医学》2002年第6期620-622,629,共4页Chinese Journal of Clinical Medicine

摘　　要：目的:探讨联合应用肠内营养(EN)、膳食纤维(diet fiber)和重组生长激素(recombinant growth hormone,rGH)治疗极短肠大鼠,及对短肠大鼠结肠形态学代偿的促进作用。方法:制成切除80％～85％短肠大鼠动物模型,用百普素行肠内营养,并加用diet fiber和rGH。随机分成三组:肠内营养组(EN),肠内营养+膳食纤维组(EF),肠内营养+膳食纤维+生长激素组(EFG),及正常大鼠对照组(CONT)。观察全身营养状况、结肠形态学、结肠上皮细胞DNA和倍体及结肠组织IGF-1和IGF-1受体mRNA含量。结果:术后第15天EFG组体重减轻明显好于EN组并于第21天体重大于术前。EFG组净氮平衡的改善明显好于EF和EN组,血浆蛋白EFG组明显高于EN组。各组间形态学差异非常显著,各实验组结肠黏膜细胞DNA含量和细胞活动周期明显高于对照组,以EFG组最高。各实验组IGF-1mRNA含量比CONT组明显增高,EFG组最高;各实验组IGF-1受体mRNA含量比CONT组明显减低,以EFG组最低。结论:1.短肠综合征时大鼠结肠发生了明显的形态和功能代偿。2.Diet fiber和rGH对短肠大鼠结肠形态学的代偿有明显促进作用,联用效果最佳。Objective: To study the treatment for the short bowel rats by combination of enteral nutrition,diet fiber and re-combinant growth hormone in the promotion of colonic morphological adaption. Methods: Twenty rats were resected 80~ 85% small intestine and randomly assigned to one of three groups: enteral nutrition group (EN), enteral nutrition,diet fiber group(EF),enteral nutrition, diet fiber and recombinant growth hormone(rGH) group (EFG). Another ten normal rats were controls (CONT). Nutritional state,colonic morphology, IGF - ImRNA and IGF - 1 - recetpor mRNA of the colon were investigated. Results: On POD 15, body weight of the EFG group was heavier than that of EN group( -4.3 ?

关 键 词：膳食纤维 短肠综合征 结肠代偿 肠内营养 重组生长激素 动物模型 

分 类 号：R574.62[医药卫生—消化系统]