蠡河底泥中反硝化复合菌群富集及菌群结构研究  

Enrichment and Characterization of A Denitrifying Bacteria Consortium from Lihe River's Sediment

作　　者：雍佳君 成小英[1] 

机构地区：[1]江南大学环境与土木工程学院,无锡214122

出　　处：《环境科学》2015年第6期2232-2238,共7页Environmental Science

基　　金：国家水体污染控制与治理科技重大专项(2012ZX0701-013-04)

摘　　要：从无锡市滨湖区蠡河底泥中富集培养反硝化复合菌群,研究其在不同富集培养阶段TN、NO-3-N、NO-2-N、NH+4-N和COD动态变化,分析反硝化过程中气体释放总量、释放速率和成分,通过构建全长16S r DNA克隆文库研究其菌落结构.结果表明,反硝化复合菌群富集在阶段4时脱氮效果最佳,仅在9 h内,330 mg·L-1的TN负荷下,TN去除率达90.9%,NO-3-N去除率达100%,中间产物NO-2-N和NH+4-N积累量最少,分别为3.39 mg·L-1和16.64 mg·L-1,COD去除率达85%;释放气体260m L,气体主要成分为N2,同时还有少量的CH4和CO2等.富集培养反硝化复合菌群细菌属于Pseudomonadaceae科和Rhodocyclaceae科,为Proteobacteria门,OUT丰度分别为57.8%和31.6%,Pseudomonadaceae科是优势类群.A denitrifying bacteria consortium was enriched from Li He River’s sediment,the dynamics of total nitrogen( TN),nitrate( NO-3-N),nitrite( NO-2-N),ammonium( NH+4-N) and COD at different enrichment cultivation stages were studied,and the total volume,the releasing rates and the composition of gas released during the denitrification process were analyzed. The full-length16 S r DNA clone library was constructed,enclosing the diversity of the denitrifying bacteria consortium. The results showed,in the enrichment phase 4,under the load of TN 330 mg·L- 1,the best nitrogen removal effect was obtained,which the TN and NO-3-N removal rates reached 90. 9% and 100% within 9 hours,respectively. The accumulation amounts of NO-2-N and NH+4-N were merely3. 39 mg·L- 1and 16. 64 mg·L- 1. And the COD removal rate was 85%. The process released 260 m L of the compound gas,in which the main ingredient was N2 associated with a small quantity of CH4 and CO2. The denitrifying bacteria consortium consisted of the family Pseudomonadaceae and the family Rhodocyclaceae,belonging to Proteobacteria phylum,in which the OUT abundances were 57. 8%and 31. 6%,respectively. The family Pseudomonadaceae was the predominant group.

关 键 词：富集培养 反硝化复合菌群 脱氮 气体释放 16S rDNA克隆文库 

分 类 号：X172[环境科学与工程—环境科学]