Renal cell carcinoma related novel gene, GYLZ-RCC18: cloning and functional studies  

肾癌特异相关新基因GYLZ-RCC18的克隆及功能研究(英文)

作　　者：张强[1] 梁丽莉[1] 席志军[1] 丁义[1] 佟明[1] 张志文[1] 郭应禄[1] 李钟[2] 陈琳 

机构地区：[1]北京大学第一医院北京大学泌尿外科研究所,北京100034 [2]美国芝加哥西北大学 [3]福建省血液研究所,厦门361001

出　　处：《Chinese Medical Journal》2002年第5期746-749,155-156,共4页中华医学杂志（英文版）

基　　金：ThisprojectwassupportedbyagrantfromtheNationalNaturalScienceFoundationofChina (No 3 9870 841)

摘　　要：OBJECTIVE: To clone the full length of renal cell carcinoma (RCC) related novel gene GYLZ-RCC18 and study its function. METHODS: SMART RACE technology was used to clone the full length of GYLZ-RCC18. RT-PCR was used to detect its expression in renal cell carcinoma tissue at different stages and grades. We transfected the antisense oligonucleotide of GYLZ-RCC18 to renal cell carcinoma cell line, GRC-1, and analyzed proliferation activity, growth rate, apoptosis, and mortality changes. RESULTS: The full length of GYLZ-RCC18 (GenBank accession number: BE825133) cDNA was about 3.5 kb. GYLZ-RCC18 had a higher expression in higher grades and stages of renal cell carcinoma than in lower ones. The expression of GYLZ-RCC18 in renal cell carcinoma was much higher than in normal kidney. After the transfection of GYLZ-RCC18 antisense oligonucleotide, the mortality of GRC-1 increased significantly, while proliferative activity and growth rate were substantially inhibited at the same time. The antisense oligonucleotide induced apoptosis of GRC-1 through the entire observation time. CONCLUSION: GYLZ-RCC18 is an important novel gene related to renal cell carcinoma. Overexpression of this gene results in higher growth and proliferative activity and has an antiapoptosis effect on renal cell carcinoma cells. Transfection of the antisense oligonucleotide may inhibit the generation and development of renal cell carcinoma.目的　克隆肾癌相关新基因GYLZ RCC18的全长 ,并探讨其功能。方法　应用SMARTRACE技术克隆GYLZ RCC18的全长 ,应用逆转录PCR的方法检测其在肾癌组织和细胞系中的表达特点 ,以及与肾癌组织分级、分期的关系 ,通过转染GYLZ RCC18的反义寡核苷酸阻断其表达 ,观察其对肾癌细胞系生命特征的变化。结果　GYLZ RCC18全长约 3 5kb(基因号 :BE82 5 133)。GYLZ RCC18在肾癌特异高表达 ,正常肾表达量极低甚至不表达 ,两者表达量差别 1 9倍之间 ;GYLZ RCC18在高分级、高分期肾癌中表达明显高于低分级、低分期肾癌的表达。转染反义基因可明显抑制GRC 1的生长、降低其增殖活性、减少核分裂、并诱导其持续性凋亡。结论　本研究表明GYLZ RCC18是肾癌发生发展密切相关的重要新的癌基因 ,它的表达与肾癌细胞的生存、增殖、抗凋亡发展密切相关 ,它的成功克隆为今后肾癌分子机制研究提供了新的理论依据。

关 键 词：Apoptosis Carcinoma  Renal Cell Cell Division Cell Line Cloning  Molecular DNA  Antisense DNA  Complementary Gene Expression Regulation  Neoplastic Genes  Neoplasm Humans Kidney Neoplasms Neoplasm Proteins OLIGONUCLEOTIDES Research Support  Non-U.S. Gov't Sequence Analysis  DNA Transfection Tumor Cells  Cultured 

分 类 号：R737.11[医药卫生—肿瘤]